| CHANG Gong-min,PENG Rui-yun,GAO Ya-bing,WANG Rui-juan,XU Xin-ping,MA Jun-jie,WANG Shui-ming.Therapeutic effect of rhIL-11 and rhG-CSF on mouse bone marrow injury induced by neutron irradiation[J].Chinese Journal of Radiological Medicine and Protection,2009,29(4):375-379 |
| Therapeutic effect of rhIL-11 and rhG-CSF on mouse bone marrow injury induced by neutron irradiation |
| Received:December 26, 2008 |
| DOI： |
| KeyWords:Neutron Mouse Bone marrow Recombinant human interleukin-11(rhIL-11) Recombinant human granulocyte colony stimulating factor(rhG-CSF) |
| FundProject:全军"十一五"医药卫生专项基金(06Z064) |
| Author Name | Affiliation | E-mail | | CHANG Gong-min | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | PENG Rui-yun | Beijing Institute of Radiation Medicine, Beijing 100850, China | Pengry@nic.bmi.ac.cn | | GAO Ya-bing | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | WANG Rui-juan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | XU Xin-ping | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | MA Jun-jie | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | WANG Shui-ming | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the therapeutic effect of rhIL-11 and rhG-CSF on mouse bone marrow injury induced by neutron irradiation. Methods 130 male BALB/c mice were irradiated by 3.0 Gy neutron and injected with rhIL-11 at 600 μg·kg－1·d－1 and rhG-CSF at 50 μg·kg－1·d－1 for 3 days after irradiation. The mice peripheral blood cells, bone marrow pathological changes, bone marrow nucleated cell counts, AgNOR content, apoptosis and necrosis rates and Bax protein content were observed by means of blood cells automatic analyzer, HE staining, AgNOR staining, flow cytometry, immunohistochemistry staining and image analysis. Results In the irradiation group and the rhIL-11 group, the mice peripheral blood white blood cells, bone marrow nucleated cell counts and AgNOR content was decreased progressively. The Bax protein was positively or strongly positively expressed in the cytoplasm of the hematopoietic cells and the Bax protein content was increased progressively at 6 h, 1 d, 3 d after irradiation. In the irradiation group, the rates of apoptosis and necrosis in the mice hematopoietic cells were greatly increased and that of necrosis was significant at 6 h after irradiation. In the rhIL-11+rhG-CSF group, the counts of bone marrow nucleated cell and AgNOR were increased and the Bax protein content was decreased at 3 d after irradiation, while in the rhIL-11 group,the indexes mentioned above were not obviously different compared with those of the irradiation group. Conclusions The mice bone marrow hematopoietic function is seriously damaged by 3.0 Gy neutron irradiation. rhIL-11 and rhG-CSF could improve the mice hematopoietic function after neutron irradiation, and combination of them is more effective to stimulate the hematopoitic function than either of them alone. |
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