| LI Yan-bo,GUO Cai-xia,DONG Li-hua,WANG Jian-feng,LIU Shu-chun,GONG Shou-liang.Effects of multiple low dose radiation on the apoptosis of splenocytes and immune factor in male diabetic rats[J].Chinese Journal of Radiological Medicine and Protection,2009,29(4):364-367 |
| Effects of multiple low dose radiation on the apoptosis of splenocytes and immune factor in male diabetic rats |
| Received:September 28, 2008 |
| DOI： |
| KeyWords:Low dose radiation Diabetes mellitus Spleen Apoptosis Immune factor |
| FundProject:国家自然科学基金(30570546) |
| Author Name | Affiliation | E-mail | | LI Yan-bo | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | GUO Cai-xia | 卫生毒理学教研室 | | | DONG Li-hua | 吉林大学第一医院肿瘤中心放疗科 | | | WANG Jian-feng | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | LIU Shu-chun | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | GONG Shou-liang | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | gongsl@163.com |
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| Abstract:: |
| Objective To explore the effect of multiple low dose radiation (LDR) on the apoptosis of splenocytes and immune factors in diabetes mellitus (DM) rats. Methods The rats were randomly divided into control, DM and DM + LDR groups. The irradiation doses were 25, 50 and 75 mGy, and the irradiated times were 15. At the fourth weekend after the DM rats irradiated, the apoptotic rate and TCRα β percentage of splenocytes were detected by flow cytometry, and the content of IL-2 in both serum and supernatant of cultured splenocytes were detected by ELISA. Results Compared with that in the control, the body weight (BW) decreased in the DM and DM+LDR groups, particularly in DM group. The blood glucose (BG) level in the DM+LDR groups was higher than that in the control, but decreased significantly as compared with that in the DM group (P<0.01). As compared with those in the control, the apoptotic rate in DM+50 mGy (P<0.05) and the content of serum IL-2 in DM+75 mGy group (P<0.01) all increased significantly, while the content of IL-2 in supernatant of cultured splenocytes decreased significantly in the DM+LDR groups. Compared with those in the DM group, the apoptotic rate and the percentage of TCRα β in splenocytes in the DM+LDR groups (P<0.01-P<0.001) and the content of IL-2 in serum in DM+50 mGy group (P<0.01) decreased significantly. Conclusions The multiple LDR could weaken the loss of BW and increase of BG caused by DM, decrease the splenocyte apoptosis induced by DM, and regulate the immune factors. |
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