| CHEN Xia-lin,CAO Jian-ping,JI Rong,ZHU Wei,LIU Yang,GONG Xiao-mei,TANG Yan,PAN Chun-yan,FAN Sai-jun.Radiation sensitization by dihydroartemisinin on human HeLa cells of cervical cancer[J].Chinese Journal of Radiological Medicine and Protection,2009,29(4):360-363 |
| Radiation sensitization by dihydroartemisinin on human HeLa cells of cervical cancer |
| Received:December 26, 2008 |
| DOI： |
| KeyWords:Dihydroartemisinin Human cervical cancer cells Radiosensitizing effect Cell cycle Apoptosis |
| FundProject:国家自然科学基金(30670632) |
| Author Name | Affiliation | E-mail | | CHEN Xia-lin | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | CAO Jian-ping | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | jpcao@suda.edu.cn | | JI Rong | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | ZHU Wei | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | LIU Yang | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | GONG Xiao-mei | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | TANG Yan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | PAN Chun-yan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | FAN Sai-jun | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | |
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| Abstract:: |
| Objective To investigate the radiosensitizing effects of dihydroartemisinin (DHA)on human HeLa cells of cervical cancer irradiated by X rays. Methods Cell growth kinetics was determined using MTT assay. Cell survival was analyzed by clonogenic assay. The change of cell cycle and apoptosis was measured by flow cytometry. Results Dihydroartemisinin inhibited the growth of HeLa cells of human cervical cancer and showed a dose-dependent and time-dependent manner. Dihydroartemisinin (20 μmol/L) showed the radiosensitizing effects on HeLa cells, and the sensitizing enhancement ratio (SER) was 1.47. Dihydroartemisinin abrogated radiation-induced G2 arrest of the tested HeLa cells, the G2 ratio of medicine+radiation group declined from 73.58% to 48.31%. Dihydroartemisinin enhanced the apoptosis of HeLa cells by X-irradiation, the apoptosis rates of medicine+radiation group significantly increased from 29.46%, 48.04%, 70.21% to 45.79%, 66.36% and 79.58%, respectively for 2, 4 and 6 Gy. Conclusions Dihydroartemisinin could increase the radiosensitivity of HeLa cells of human cervical cancer. Abrogation of radiation-induced G2 arrest could be part of the mechanism. |
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