| CUI Yu-fang,JIANG Zhu-jun,DU Li,MA Qiong,LIU Xiao-lan,XIE Ling,DONG Bo,MAO Jian-ping,MAN Qi-hang,ZHANG Chao.Analysis of the transcription profiles of mouse thymus irradiated by γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2009,29(4):355-359 |
| Analysis of the transcription profiles of mouse thymus irradiated by γ-rays |
| Received:December 18, 2008 |
| DOI： |
| KeyWords:Ionizing radiation Thymus Gene chips Cell cycle Immune stress Apoptosis |
| FundProject:国家科技重大专项 |
| Author Name | Affiliation | E-mail | | CUI Yu-fang | Beijing Institute of Radiation Medicine, Beijing 100850, China | yufangc@vip.sina.com | | JIANG Zhu-jun | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | DU Li | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | MA Qiong | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | LIU Xiao-lan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | XIE Ling | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | DONG Bo | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | MAO Jian-ping | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | MAN Qi-hang | 辽宁医学院校外培训基地航天中心医院 | | | ZHANG Chao | 辽宁医学院校外培训基地航天中心医院 | |
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| Abstract:: |
| Objective To investigate the dynamic changes of transcription profiles of mouse thymus gene expression in different times after 6 Gy γ-irradiation. Methods High-flux cDNA microarray technique was used to detect the changes in gene expression of irradiated mouse thymus. Results 1 With time-lapse after irradiation, the numbers and types of differentially expressed genes were gradually decreased, for instance, the gene numbers were 878, 318, 162 and 25 at 1, 6, 14 and 28 d after irradiation, respectively. 2Radiation-induced differential expression genes were involved in cell cycle, immunity and stress, apoptosis, signal transduction, transcription regulation, DNA synthesis and recombination, cystoskeleton, ion channel and transportation, metabolism, protein translation and synthesis, development and cell differentiation, etc. 3 Analysis of some important differential expression genes after irradiation showed that there were 5 genes correlated cell cycle (3 up-regulating:Cyclin G,Anxa1,Fgf1 and 2 down-regulating:Cdc2a, Cdc25b), 5 genes correlated immune stress(4 up-regulating:IL-18, Casp1, IL-15, IL-7 and 1 down-regulating:Cd28), 7 genes correlated apoptosis(4 up-regulating:Casp1, Anxa1, Perp, IL-7 and 3 down-regulating:Pten, Api5 and Fas). Conclusions After 6 Gy irradiation, differentially expressed genes in mouse thymus is not only involved in many targets, levels and pathways, but also displayed an obvious difference in times. This reveals the regular pattern of differential expression genes in the process of injury and reconstitution in moderate dose irradiated mouse thymus. |
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