| ZONG Zhao-wen,CHENG Tian-min,Ran Xin-ze,et al.CXCR4 gene transfection enhancing the distribution of dMSCs to the wounded skin of rats with combined wound and irradiation injury[J].Chinese Journal of Radiological Medicine and Protection,2009,29(4):351-354 |
| CXCR4 gene transfection enhancing the distribution of dMSCs to the wounded skin of rats with combined wound and irradiation injury |
| Received:January 08, 2009 |
| DOI： |
| KeyWords:CXCR4 Gene transfection Dermal multipotent stem cells Combined injury |
| FundProject:国家重点基础研究发展规划资助(973)项目(2005CB522605);国家自然科学基金(30500141) |
| Author Name | Affiliation | | ZONG Zhao-wen | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | CHENG Tian-min | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | Ran Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | SU Yong-ping | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | DONG Shi-wu | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | LI Nan | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | WANG Jun-ping | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | AI Guo-ping | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | LI Zheng | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To observe whether the transplanted dermal multipotent stem cells (dMSCs) transfected by adenovirus vector of CXCR4 (Adv-CXCR4) can distribute more frequently to the wound of rats with combined wound and irradiation injury. Methods dMSCs transfected by Adv-CXCR4 (group A), or transfected by adenovirus vector of green fluorescent protein (group B), and non-transfected dMSCs were labeled with 3H-TdR and then transplanted into combine-injured rats. The amount of dMSCs in wound were determined by liquid scintillation, and wounds healing process was observed by measuring the remaining wound area. Results From the 5th day after transplantation, the amount of dMSCs in the wound of group A accounted for 1.95%-3.85% of the total transplanted dMSCs, significantly greater than those in group B and group C, which accounted for 1.07%-1.86% of the total transplanted dMSCs. The remaining wound area in group A was smaller than those in group B and group C from day 12 after injury, and the healing time of group A was 1.5 day ahead than group B and group C. Conclusions dMSCs transfected by Adv-CXCR4 distributes more frequently to the wound of combine-injured rats and could accelerate wound healing. |
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