| LI Chang-feng,ZHANG Bin,WANG Fang,et al.Effects of RNAi targeting survivin gene on A549 cell radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2009,29(3):268-270 |
| Effects of RNAi targeting survivin gene on A549 cell radiosensitivity |
| Received:June 02, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.03.005 |
| KeyWords:RNAi Survivin Apoptosis Radiosensitivity |
| FundProject:吉林省科技厅资助项目(200705274) |
| Author Name | Affiliation | E-mail | | LI Chang-feng | Department of Endoscopy, China-Japan Union Hospital of Jilin University, Changchun 130033, China | | | ZHANG Bin | Department of Endoscopy, China-Japan Union Hospital of Jilin University, Changchun 130033, China | binzhang60091@yahoo.com.cn | | WANG Fang | Department of Endoscopy, China-Japan Union Hospital of Jilin University, Changchun 130033, China | | | WANG Zhi-cheng | 吉林大学公共卫生学院卫生部放射生物学重点实验室 | | | YANG Hai-shan | Department of Endoscopy, China-Japan Union Hospital of Jilin University, Changchun 130033, China | |
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| Abstract:: |
| Objective To construct the RNAi vector targeting survivin gene, in order to observe its effect on lung adenocarcinoma A549 cell radiosensitivity, and explore the possible mechanism.Methods One pair of RNA interference sequence targeting survivin gene was designed according to the cDNA sequence, the recombinant RNAi plasmid pGenesil2-survivin was constructed. After certified by enzyme digestion and sequencing, the pGenesil2-survivin plasmid was transfected into A549 cells. Survivin mRNA and protein was measured by RT-PCR and Western blot, respectively. The apoptosis was analyzed with flow cytometry. The radiosensitivity was measured by clone formation assay.Results The pGenesil2-survivin vector was successfully established. After the pGenesil2-survivin was transfected into A549 cells for 48 h, surviving protein and mRNA in pGenesil2 group were not obviously changed compared with that in normal group. They were increased after 5 Gy X-ray irradiation, but obviously inhibited in pGenesil2-survivin group. The cell apoptosis in pGenesil2-survivin and 5 Gy X-ray groups was obviously increased (t1=10.63,P<0.001;t2=3.75 ,P<0.05), the effect was more manifest in combined group(t=4.83,P<0.05). D0 and Dq in normal and pGenesil2 group were not obviously different, but those in pGenesil2-survivin group were lower.Conclusions RNAi targeting survivin gene could inhibit survivin mRNA and protein expression, enhance the A549 cell apoptosis rate and cell radiosensitivity. |
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