| XU Xiao-ya,JIN Wei-fang,WANG Hong-fu,GAO Jian-jun.Earlier pathological character and mechanisms of irradiation necrosis on femoral head[J].Chinese Journal of Radiological Medicine and Protection,2009,29(3):264-267 |
| Earlier pathological character and mechanisms of irradiation necrosis on femoral head |
| Received:May 27, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.03.004 |
| KeyWords:Irradiation Osteoncrosis of femoral head Osteoradionecrosis Empty bone lacuna Bone marrow mesenchymal stem cell |
| FundProject:上海市卫生局基金(08GWZX0602);上海市卫生局青年科研基金(054y27) |
| Author Name | Affiliation | E-mail | | XU Xiao-ya | Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | JIN Wei-fang | Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | mulei80@sina.com | | WANG Hong-fu | Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | GAO Jian-jun | Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | |
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| Abstract:: |
| Objective To observe the earlier pathological character and mechanism of radiation osteonecrosis in femoral head, in order to provide evidences for the earlier diagnosis and prevention of radiation osteonecrosis of femoral head.Methods Single femoral head of rats were irradiated singly with 30 Gy of 137Cs γ-ray. The rats were executed after 2, 6 and 12 weeks, then the femurs were stained with HE and histopathological changes were observed by light microscope. The bone marrow mesenchymal stem cells (BMSCs) were cultured after 2 weeks and its proliferation and the colony formation were observed. The rats were endo-perfused with microfill contrast medium 12 weeks later, and the 3-dimensional structure of capillaries by Micro-CT was re-established to detect the pathological changes of capillaries after irradiation.Result The irradiated femur showed deranged chondrocyte, decreased osteocyte, shrinking nucleus, increased empty bone lacuna and reduced bone trabecula (P<0.05). Micro-CT showed the discontinued small vessels and absence(6.65%) capillaries in irradiated femur were obviously less than those of the unirradiated (12.3%)(P<0.001). The proliferation of BMSCs was slowed, the number of colony in irradiated group (10%) was less than that of control (21%) (P<0.001).Conclusions The preliminary histopathological changes of osteoradionecrosis on femoral head could be increased the empty bone lacuna, and the bone lacuna above 30% was the sign of the earlier period of osteoradionecrosis. The osteonecrosis of femoral head induced by radiation is not only correlated to the damages to the bone, but also to the damages to BMSCs and capillaries. |
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