| YANG Huan,XU Guo-xu,LIU Dong-wei,LIU Yu-long,PU Shu-yang,JI Xiao-yan.Expression of αB-crystallin in X-ray irradiated rat lenses[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):160-162 |
| Expression of αB-crystallin in X-ray irradiated rat lenses |
| Received:October 06, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.011 |
| KeyWords:αB-crystallin Radiation-induced cataract Molecular chaperone |
| FundProject: |
| Author Name | Affiliation | E-mail | | YANG Huan | Department of Ophthalmology, Second Hospital Affiliated to Soochow University, Suzhou 215004, China | | | XU Guo-xu | Department of Ophthalmology, Second Hospital Affiliated to Soochow University, Suzhou 215004, China | phacoxu@sohu.com | | LIU Dong-wei | Department of Ophthalmology, Second Hospital Affiliated to Soochow University, Suzhou 215004, China | | | LIU Yu-long | 215004 苏州大学附属第二医院肿瘤科 | | | PU Shu-yang | Department of Ophthalmology, Second Hospital Affiliated to Soochow University, Suzhou 215004, China | | | JI Xiao-yan | Department of Ophthalmology, Second Hospital Affiliated to Soochow University, Suzhou 215004, China | |
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| Abstract:: |
| Objective To detect the changes of soluble αB-crystallin in X-ray irradiated rat lens, and to explore the potential role of αB-crystallin in the pathogenesis of radiation-induced cataract.Methods The radiation cataract model was established with the accelerator linear in male Sprague-Dawley (SD) rats. The rats were divided into normal control group, experimental control group and X-irradiated group (the doses were 5, 15, and 25 Gy, respectively). The rats were killed at 3 months post-irradiation, and the lenses were carefully isolated and homogenized. The protein changes of αB-crystallin in lens supernatant were measured by Western blotting.Results Typical radiation-induced cataract was observed in 15 and 25 Gy groups, while the lenses of the normal control group, experimental control group and the 5 Gy irradiation group remained transparent. The αB-crystallin protein level was significantly decreased in a dose-dependent manner (5 Gy group:0.871±0.085;15 Gy group:0.643±0.096;25 Gy group:0.338±0.160;F=40.764, P<0.05).Conclusion The decreased expression of the molecular chaperone αB-crystallin in X-irradiated rat lens indicates its important role in the pathogenesis of irradiation cataract. |
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