| BAO Peng-tao,QI Hao-wen,GAO Wei,et al.Effect of ulinastatin on tumor necrosis factor and interleukin-6 in rats with radiation-induced lung injury[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):154-156 |
| Effect of ulinastatin on tumor necrosis factor and interleukin-6 in rats with radiation-induced lung injury |
| Received:April 17, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.009 |
| KeyWords:Ulinastatin TNF-α IL-6 Lung Radiation |
| FundProject: |
| Author Name | Affiliation | E-mail | | BAO Peng-tao | Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China | bao03@163.com | | QI Hao-wen | Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China | | | GAO Wei | 唐都医院呼吸内科 | | | 李树钧 | Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China | | | 张力江 | Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China | |
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| Abstract:: |
| Objective To observe the effect of ulinastatin on tumor necrosis factor(TNF-α) and interleukin-6(IL-6)in radiation-induced lung injury.Methods Seventy-two female SD rats were randomly divided into 3 groups as control group,irradiation group and treatment group (administered with Ulinastatin).Rats in irradiation group and treatment group were irradiated with linear accelerator at a single dose of 25 Gy.After irradiation rats in treatment group were injected daily with ulinastatin at a dose of 100 000 U·kg－1·d－1 for 7 days through caudal vein while rats in control group and irradiation group were injected with the same volume of saline. Rats were killed at 2 h,4,8 and 24 weeks. Samples of lung tissues were observed by using HE staining.Expression of TNF-α in lung was determined by Western blot and expression of IL-6 in serum was determined by ELISA. Data were analyzed by SPSS software.Results Expressions of TNF-α in lung and IL-6 in serum increased significantly after irradiated in irradiation group compared with control group, and it reached the peak at 4 weeks(q=5.63、6.21,P<0.01).Though expressions of TNF-α and IL-6 in treatment group also increased compared with control group,the difference between irradiation group and treatment group was statistic significantly (q=4.97、7.42,P<0.01).Conclusion TNF-α and IL-6 play an important role in radiation-induced lung injury. Ulinastatin could suppress the inflammatory response and radiation-induced lung injury effectively by decreasing the levels of TNF-α and IL-6. |
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