| HOU Dian-jun,MA Ya,LIU Wei,et al.X-ray induced TCR gene mutation of peripheral blood lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):151-153 |
| X-ray induced TCR gene mutation of peripheral blood lymphocytes |
| Received:April 01, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.008 |
| KeyWords:Radiation exposure Gene mutation TCR gene Biological dosimeter |
| FundProject:山东省自然科学基金(Y2007C151) |
| Author Name | Affiliation | | HOU Dian-jun | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | MA Ya | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | LIU Wei | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | QIAO Jian-wei | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | LU Feng | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | SHANG Xi-mei | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | LI Jie-qing | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China | | FENG Li | Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China |
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| Abstract:: |
| Objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X-ray exposure using cultured lymphocytes cloning method.Methods Freshly isolated peripheral lymphocytes from healthy adult donors were irradiated with X-ray in doses ranging from 0 to 8 Gy and cultured with interleukin2 and phytohemagglutinin for 7 days. The mutant frequencies of TCR gene (TCR MF) were detected by flow cytometry and the dose response curves were fitted.Results TCR MF increased with the dose going up. An aquadratic polynomial dose response model was fitted.Conclusion TCR gene mutation could which serve as a potential biological dosimeter. It might be applied for the estimation of biological dose in emergency exposure. |
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