| HUANG Bo,CHEN Feng,YANG Zhi-hua.Oxidative damage of BEAS-2B cells induced by depleted uranium and protection by DMSO[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):143-146 |
| Oxidative damage of BEAS-2B cells induced by depleted uranium and protection by DMSO |
| Received:June 26, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.006 |
| KeyWords:Depleted uranium BEAS-2B cell Reactive oxygen species Antioxidant enzyme DMSO |
| FundProject:国家自然科学基金(30271524);全军十五指令性课题(L061) |
| Author Name | Affiliation | E-mail | | HUANG Bo | Institute of Radiation Medicine, Beijing 100850, China | zhumx@nic.bmi.ac.cn | | CHEN Feng | 南华大学 | | | YANG Zhi-hua | Institute of Radiation Medicine, Beijing 100850, China | | | 潘秀颉 | Institute of Radiation Medicine, Beijing 100850, China | | | 曹珍山 | Institute of Radiation Medicine, Beijing 100850, China | | | 朱茂祥 | Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To observe the oxidative damage in human bronchial epithelial cells (BEAS-2B) induced by depleted uranium (DU) and protection of DMSO.Methods The measurement of extracellular superoxide anions (O2-·) was based on the reduction of ferricytochrome C. Quantitative analysis of extracellular hydrogen peroxides (H2O2) was used by the horseradish peroxidase-dependent oxidation of phenol red. The determination of extracellular hydroxyl radicals (·OH) was based on discoloration of safranine T. Ethidium bromide and 2,7'-dichlorofluorescein, fluorescent products of the membrane-permeable dyes-hydroethineand 2,7'-dichloroflurescin diacetate were used to monitor the intracellular production of O2-· and H2O2 by fluorometric method. The enzyme activity of SOD and GSH were measured by chemiluminescence and spectrophotometric method, respectively.Results The ROS production, including H2O2, O2-· and ·OH, increased remarkably which induced by DU in BEAS-2B cells. The enzyme activity of SOD and GSH was descended remarkedly. These changes could be effectively inhibited by 0.5% of DMSO.Conclusion DU causes oxidative damage to BEAS-2B cells.Through removing active oxygen,DMSO can inhibit oxidative damage of DU. |
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