| LIU Shui-bing,HU Pei-zhen,HOU Ying,LI Xu-bo,TIAN Qiong,SHI Mei.Mechanism of recombinant human bone morphogenetic protein-2 in repairing hematopoietic injury in mice exposed to γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):139-142 |
| Mechanism of recombinant human bone morphogenetic protein-2 in repairing hematopoietic injury in mice exposed to γ-rays |
| Received:April 16, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.005 |
| KeyWords:Bone morphogenetic protein Hematopoiesis Radiation injury SRY gene DNA probe |
| FundProject:国家自然科学基金(30470528) |
| Author Name | Affiliation | E-mail | | LIU Shui-bing | Department of Pathology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China | tqiong@fmmu.edu.cn | | HU Pei-zhen | 710032 西安, 第四军医大学药学系病理学教研室 | | | HOU Ying | Department of Pathology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China | | | LI Xu-bo | Department of Pathology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China | | | TIAN Qiong | Department of Pathology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China | | | SHI Mei | 西京医院 | |
|
| Hits: 2697 |
| Download times: 1961 |
| Abstract:: |
| Objective To investigate the mechanism of recombinant human bone morphogenetic protein-2(rhBMP-2) in repairing hematopoietic injury in mice irradiated with γ-ray. To prepare SRY gene probe and study the effect of rhBMP-2 in repairing hematopoietic injury in mice by in situ hybridization.Methods Twenty-two BALB/c female mice were randomly divided into the irradiated group and BMP treated group, respectively. Bone marrow cells of normal male mice were transplanted into 22 female mice post-irradiation to 8.5 Gy of 60Coγ rays. The left femurs of the survived female mice were re-irradiated with 9 Gy 14 days later. Mice in BMP treated group were given rhBMP-2 20 mg/kg while those in control group were treated with 0.9% saline by intraperitoneal injection every day for 6 days. These mice were killed 14 days later and paraffin sections of femurs were made. The SRY gene was detected with in situ hybridization.Results There were more positive blots in the left femurs of the mice in irradiated group than those in BMP treated group(T=155.0,P<0.05). The number of positive blots between the left femurs of the mice in irradiated group and the right femurs of the mice in two groups was not significantly different(T=92.0,78.5,P>0.05). The number of positive blots in the left femurs of the mice in BMP treated group was significantly less than those in the right femurs of the mice in two groups(T=155.0,55.0,P<0.05).Conclusion No donor cell of male mice was detected in the left femurs of BMP treated group, suggesting that rhBMP-2 promoted the restoration of residuary bone marrow cells. Thus, rhBMP-2 promotes the proliferation or differentiation of residuary mesenchymal stem cells, improves hematopoietic microenvironment and accelerates the hematopoietic restoration. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
