| ZHENG Huai-en,WANG Feng-chao,RAN Xi,HAN Jing,CHEN Jun.Protective effect of atorvastatin on radiation-induced endothelial cell injury[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):129-132 |
| Protective effect of atorvastatin on radiation-induced endothelial cell injury |
| Received:November 03, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.002 |
| KeyWords:Radiation injury Blood vessel endothelial cell (VEC) Atorvastatin Thrombomodulin (TM) Protein C |
| FundProject:国家自然科学基金(30770642);重庆市自然科学基金(CSTC 2004BB5040) |
| Author Name | Affiliation | E-mail | | 冉新泽 | Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | huaienzheng@hotmail.com | | ZHENG Huai-en | Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | WANG Feng-chao | Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | RAN Xi | 400038 重庆, 第三军医大学学员旅21队 | | | 王艾平 | Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | HAN Jing | Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 张彦琦 | 400038 重庆, 第三军医大学卫生统计学教研室 | | | CHEN Jun | 400038 重庆, 第三军医大学卫生统计学教研室 | |
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| Abstract:: |
| Objective To explore the protective effect of atorvastatin on irradiated endothelium and the thrombomodulin (TM) expression.Methods Cultured human coronary artery endothelial cells (HCAEC) and human umbilical vein endothelial cells (HUVEC) were treated by atorvastatin at the final concentration of 10 μmol/ml for 10 min, and then irradiated with 2 and 25 Gy. Cell cycles status and TM expression were quantitatively measured by flow cytometry 24 hours after irradiation. Protein C activation in endothelial cells was also assessed.Results After administration with atorvastatin for 24 h, the TM expression increased by 77%, 59% and 61% in normal control group, 2 Gy group and 25 Gy group, respectively (t=27.395,26.420,58.065;P=0.000).The protein C levels decreased by 23% and 34% compared with the normal group post_irradiation to 2 and 25 Gy, but increased by 79% and 76% compared with the irradiated control group after administration with atorvastatin. The rates of cell apoptosis decreased by 6% and 16% in 2 Gy and 25 Gy groups, respectively after administration with atorvastatin for 24 h(t=4.178,17.863;P=0.000).Conclusion Atorva statin can protect endothelia cell from irradiation-induced apoptosis by increasing TM expression and protein C activation. |
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