| LI Yu-wen,FENG Jiang-bin,LU Xue,et al.Preliminary detection of expression changes of human mitochondrial COXI, ND1 and ND6 gene induced by 60Coγ-rays[J].Chinese Journal of Radiological Medicine and Protection,2009,29(2):125-128,142 |
| Preliminary detection of expression changes of human mitochondrial COXI, ND1 and ND6 gene induced by 60Coγ-rays |
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| DOI：10.3760/cma.j.issn.0254-5098.2009.02.001 |
| KeyWords:60Coγ-rays Human lymphoblastoid cell line Mitochondrion Gene expression |
| FundProject:国家自然科学基金(30870749, 30570551);北京市自然科学基金(7053073) |
| Author Name | Affiliation | E-mail | | LI Yu-wen | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | FENG Jiang-bin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | LU Xue | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陈德清 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 刘青杰 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | qjliu@nirp.cn |
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| Abstract:: |
| Objective To explore the changes of human mitochondrial COXI, ND1 and ND6 genes expression induced by ionizing irradiation.Methods Changes of human COXI, ND1 and ND6 gene expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines, which were exposed to 1-10 Gy 60Coγ-rays. And the dose-effect relationships between expression changes of the genes and the doses were analyzed. The changes of these three genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect.Results The expression of three genes COXI, ND1 and ND6, showed either the dose-effect or the time-effect after irradiation. The gene expression levels of three genes up-regulated generally and the peak change time-point was 4 h after irradiation.Conclusion Ionizing radiation might induce the changes of mitochondrial gene expression, and the gene expression level is up-regulated. |
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