| YAO Bo,LI Yu-fang,LIU Guang-xian,HUANG Shan.A dose-effect curve of premature condensation chromosome ring in lymphocytes of human peripheral blood exposed to high dose of 60Co γ-rays in vitro[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):54-56 |
| A dose-effect curve of premature condensation chromosome ring in lymphocytes of human peripheral blood exposed to high dose of 60Co γ-rays in vitro |
| Received:June 08, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.016 |
| KeyWords:High dose radiation Premature condensation chromosome ring Dose-effect curve |
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| Author Name | Affiliation | | YAO Bo | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China | | LI Yu-fang | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China | | LIU Guang-xian | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China | | HUANG Shan | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China | | 蒋本荣 | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China | | 艾辉胜 | Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract:: |
| Objective To establish a dose-effect curve of premature condensation chromosome ring(PCC-R)in lymphocytes of human peripheral blood after exposed to high doses of γ-rays. Methods Peripheral blood samples was drawn from three healthy individuals, and exposed to 60Co γ-rays with doses between 0 and 30 Gy. The frequencies of PCC-R in premature condensation chromosome (PCC) cells obtained by Okadaic acid(OA) induction were calculated, and a dose_effect curve was fitted.Results PCC index tapered with dose. Frequencies of PCC-R per cell increased until 20 Gy,and then saturation was observed. The results were fitted to a lineal model up to 20 Gy:y=-0.020+0.052D,where y was the frequencies of PCC-R per cell,D was the radiation dose(Gy). Conclusion The highest dose could be estimated is 20 Gy by the dose-effect curve established with PCC-R method. Its utility and validity will be verified in the future application of radiation accident. |
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