| WAN Jian-mei,FAN Wo,ZHANG You-jiu,et al.Killing effect of 125I-UdR on human lymphoma Raji and Daudi cell lines[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):50-53 |
| Killing effect of 125I-UdR on human lymphoma Raji and Daudi cell lines |
| Received:June 08, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.015 |
| KeyWords:Lymphoma cells Raji Daudi 125I-UdR Uptake Killing effect |
| FundProject: |
| Author Name | Affiliation | | WAN Jian-mei | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | FAN Wo | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | ZHANG You-jiu | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | ZHU Ran | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | 俞泽阳 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China |
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| Abstract:: |
| Objective To evaluate the killing effect and the uptake of 125I-UdR on human lymphoma Raji and Daudi cell lines. Methods The amount of 125I-UdR in the cells and cell nuclei were determined after incubation of different time in RPMI 1640 culturing medium containing different concentrations of 125I-UdR. The killing effects of 125I-UdR on Raji and Daudi cell lines were estimated through MTT assay and cell cycle was analyzed by propidium iodide (PI) staining. Results The amounts of 125I-UdR in Raji and Daudi cells and cell nuclei were much higher than that of Na125I(P<0.05). The amounts of 125I-UdR in Raji and Daudi cells were 14 414±95 and (6916±53.69) Bq/106cell when the concentration was 100 kBq/ml. The amounts of Na125I were 68±3.8 and (324±32.8) Bq/106cell. The uptake of 125I-UdR in Raji and Daudi cells and cell nuclei increased with the 125I-UdR concentration and incubated time. The cell surviving fractions of 125I-UdR groups was much lower than that of Na125I groups (P<0.05). When the concentration was 500 kBq/ml and incubated time was 48 hours, the Raji and Daudi cell surviving fractions of 125I-UdR groups were (19.78±1.39)% and (43.17±2.69)%; those of Na125I groups were(79.10±1.79)% and (80.36±6.12)%. The surviving fractions of 125I-UdR groups reduced with the 125I-UdR concentration.Conclusion 125I-UdR can be specially ingested by Raji and Daudi cells and incorporated into DNA, then the cells will be killed. The uptake of 125I-UdR is dose and time dependent. |
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