| XIA Xin-she,ZHE Hong,MA Jian-ping,et al.Study on 3-aminobenzamide enhancing the radiosensitivity of human esophageal carcinoma cell strain in vitro[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):46-50 |
| Study on 3-aminobenzamide enhancing the radiosensitivity of human esophageal carcinoma cell strain in vitro |
| Received:October 28, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.014 |
| KeyWords:Esophageal carcinoma cell strain Radiosensitizer Poly(ADP-ribose) polymerase(PARP) Inhibitor 3-Aminobenzamide(3-AB) |
| FundProject: |
| Author Name | Affiliation | E-mail | | XIA Xin-she | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | | | ZHE Hong | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | 123zhehong@sina.com | | MA Jian-ping | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | | | YE Hong-qiang | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | | | DING Zhe | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | | | YU Wen-jun | Department of Radiotherapy, Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China | |
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| Abstract:: |
| Objective To evaluate the ability of 3-AB to sensitize the human esophageal carcinoma cell strain (CaEs-17) to radiation in vitro and its mechanisms. Methods CaEs-17 cells were treated with 3-AB at 0, 2.5, 7.5 mmol/L and given irradiation 0, 3, 6, 9, 12 Gy. 3-AB concentration in each group was made dose-survival curve using multi-target single-hit maths model by clonogenic assay. MTT assay was performed to observe the survival of irradiated cells. Comet assay and metaphase chromosome analysis were used to measure the DNA damage degree and chromosome aberration of CaEs-17 cell after 3-AB treatment and irradiation. Results Cell survival experiments showed SER of 1.21, 1.52 for 2.5 mmol/L, 7.5 mmol/L 3-AB respectively using multi-target single-hit maths model. The survival fraction of irradiated CaEs-17 cell was decreased after 3-AB treatment. DNA damage and the chromatid breakage number of irradiated CaEs-17 cells were increased after 3-AB treatment.Conclusion 3-AB, a PARP inhibitor, can enhance the radiosensitivity of human esophageal carcinoma cell strain (CaEs-17). DNA damage repair inhibition by 3-AB might be one of the mechanisms. |
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