| YANG Qing-shan,WANG Yong,FAN Fei-yue.Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):44-46 |
| Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells |
| Received:March 17, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.013 |
| KeyWords:shRNA Ku80 Radiosensitivity Cell cycle |
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| Abstract:: |
| Objective To study the role of Ku80 in the treatment of esophageal cancer through inhibiting the Ku80 expression by shRNA. Methods shRNA-Ku80 vector was constructed. The effectiveness and feasibility of RNA interference were confirmed by Western blot and RT-PCR methods. The cell sensitivity to gamma-rays was studied by colony formation assay. The effects of shRNA-Ku80 on cell cycle were observed by flow cytometry analysis. Results ShRNA-Ku80 vector was constructed successfully. Inhibition of Ku80 expression by shRNA enhanced the sensitivity of esophageal cancer cells to gamma-rays. shRNA K3 or shRNA H2 showed higher percentage of cells in G2/M phase (61.8% vs 28.6%; 64.3% vs 28.6%). Conclusion Inhibitions of Ku80 expression by shRNA play a role in the treatment of esophageal cancer. Ku80 might be a new target of tumor treatment. |
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