| ZHU Xin-hua,LIU Yue-hui.Effects of intranasal corticosteroids on radiated nasal mucosa of guinea pig[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):31-34 |
| Effects of intranasal corticosteroids on radiated nasal mucosa of guinea pig |
| Received:April 24, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009-01.009 |
| KeyWords:Nasal mucosa Ionizing radiation Corticosteroids Histopathology Cytokine |
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| Abstract:: |
| Objective To investigate a mechanism protected radiation-induce injure for radiated guinea pigsnasal mucosa treated with intranasal corticosteroids(fluticasone nasal cavity spray).Methods 50 health guinea pigs were divided into 2 groups randomly: the irradiated group (control group) with 25 guinea pigs and the administration group after irradiation (test group)with 25 guinea pigs. The nasal part of all guinea pigs were performed irradiation by the 6 MV X-ray with single 5 Gy, one time each week for three weeks. The guinea pigs of test group received intranasal corticosteroids with one time every day and one spray each side nasal cavity on the second day after three weeks irradiation. Five guinea pigs in each group were sacrificed randomly at 1 week, 1 month, 2 months, 3 months and 4 months after irradiation, and the histopathologic changes were observed under optical microscope and electron microscope. At the same time, blood were drawn from the heart and the concentration of IL-1,IL-6 and TNF-α in serum were measured by ELISA.Results The early nasal mucosa inflammatory reaction of the test group was less than the control group. The coverage rate of cilia of the test group was much than that of the control group (72.9% vs 50.2%) at four months after irradiation. The atrophy of submucosal glandular organ was lessened and they displayed some extent secretory function. The concentration of IL-1 in serum was increased very much in the test group compared with the control group after irradiation and kept higher level in the first two months. After two months, it began to decrease;on four months, it still kept equivalency level with the control group. The concentration of IL-6 and TNF-α in serum were reduced all the while.Conclusion The intranasal corticosteroids with fluticasone nasal cavity spray can reduce radiation-induced injury of guinea pigs nasal mucosa. The concentration change of IL-1, IL-6 and TNF-α in serum may be one of mechanism protected radiation-induce injure of guinea pigs nasal mucosa. |
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