| TU Xu-min,GUO Xian-hua,RUAN Jian-lei,et al.Experimental study on bystander effects K562cells induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):20-22 |
| Experimental study on bystander effects K562cells induced by ionizing radiation |
| Received:June 02, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.006 |
| KeyWords:60Co γ-rays Bystander effect K562 cell Apoptosis |
| FundProject:国家自然科学基金重点课题资助项目(10335050) |
| Author Name | Affiliation | E-mail | | TU Xu-min | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | GUO Xian-hua | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LEI Su-wen | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | Lei-suwen@163.com | | LU Hui-min | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LI Wen-jian | 中国科学院近代物理研究所 | | | WANG Ju-fang | 中国科学院近代物理研究所 | |
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| Abstract:: |
| Objective To study the bystander effects and associated mechanisms through irradiated conditioned medium(ICM).Methods Natural killer(NK) cells were obtained from peripheral blood samples. ICM irradiated with different doses of 60Co γ-rays was used for culturing K562 cell strain. The degree of injury of K562 cells by activated NK cells was observed, as well as the apoptosis frequency of K562 cell was investigated.Results Severe injury was induced in K562 cells cultured in ICM than the control (sham-irradiated) as shown by increased sensitivity to NK cells (P<0.05). The apoptosis frequency of K562 cell was increased significantly compared with the control cells(P<0.05).Conclusion The bystander effect induced by irradiation is existent. ICM can trigger the bystander effect on K562 cell strains. |
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