| LI Rong,REN Jiong,LENG Yan-bing,LIU Jing,AI Guo-ping,XU Hui.Effect of long-term depleted uranium ingestion on testosterone production and the expression of StAR and P450scc mRNAs in rats[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):13-16 |
| Effect of long-term depleted uranium ingestion on testosterone production and the expression of StAR and P450scc mRNAs in rats |
| Received:June 10, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.004 |
| KeyWords:Depleted uranium Reproduction toxicity Hormone Steroidogenic acute regulatory protein Cytochome P450 cholesterol side chain lyase |
| FundProject:国家自然科学基金资助项目(30670625);中国博士后科学基金资助项目(20060390680) |
| Author Name | Affiliation | | LI Rong | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | REN Jiong | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | LENG Yan-bing | 成都医学院公共卫生学教研 | | LIU Jing | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | AI Guo-ping | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | XU Hui | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 粟永萍 | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 程天民 | State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To explore the effect of long-term depleted uranium (DU)ingestion on testosterone production in rats, and its involvement mechanism. Methods Male and female rats (F0 and F1 generations) were exposed to DU in food at dose of 4 and 40 mgDU·kg－1·d－1 (low and high-dose groups, respectively) for 160 days, respectively. The contents of testosterone (T), luteinizing hormone (LH), and follicle stimulating hormone (FSH) in serum were detected in 20 months of F0 generations, and 15 months of F1 generations. RT-PCR was used to analyze the levels of StAR mRNA and P450scc mRNA.Results Compared with the normal control group, the testosterone contents in exposed F0 and F1 generations increased, the lowest was 51.73 U/L, but those of LH and FSH decreased. The expression of StAR mRNA in the low-dose group of F1 generation (StAR/β-actin=1.35) was up-regulated, down-regulated for other groups. Compared with the normal control group (P450scc/β-actin=0.313),the expression of P450scc mRNA in the low- and high-dose groups of F0 generation were decreased (P450scc/β-actin=0.21), and those in the low- and high-dose groups of F1 generation were increased (P450scc/β-actin=0.623) (P≤0.01).Conclusion Long-term DU exposure inhibit the male reproduction by intervening the sexual hormone production through down-regulated the expression of StAR mRNA and P450scc mRNA. |
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