| ZHANG Jiang-hong,Prise KM,JIN Yi-zun.Radiation induced bystander effect on hepatoma HepG2 cells under hypoxia condition[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):9-12 |
| Radiation induced bystander effect on hepatoma HepG2 cells under hypoxia condition |
| Received:October 28, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.003 |
| KeyWords:Hypoxia Hepatoma cell Radiation-induced bystander effect Micronucleus Signaling factor |
| FundProject:国家自然科学基金资助项目(30670629,30770644);教育部新世纪优秀人才支持计划资助项目(NCET-06-0365);上海市浦江人才计划资助项目(06PJ14012) |
| Author Name | Affiliation | E-mail | | ZHANG Jiang-hong | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Prise KM | London, Gray Cancer Institute, Oxford University, HA62JR | | | JIN Yi-zun | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | 邵春林 | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | clshao@shmu.edu.cn |
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| Abstract:: |
| Objective To investigate radiation induced bystander effect and its mechanism on hepatoma HepG2 cells under hypoxia condition.Methods Non-irradiated bystander hepatoma cells were co-cultured with irradiated cells or treated with the conditioned medium (CM) from irradiated cells, then micronuclei (MN) were measured for both irradiated cells and bystander cells.Results The MN yield of irradiated HepG2 cells under hypoxic condition was significantly lower than that under normoxia, the oxygen enhancement ratio of HepG2 cells of MN was 1.6. For both hypoxic and normoxic condition, the MN yield of bystander cells were obviously enhanced to a similar high level after co-culturing with irradiated cells or with CM treatment, and it also correlated with the irradiation dose. When the hypoxic HepG2 cells were treated with either DMSO, a scavenger of reactive oxygen species (ROS), or aminoguanidine, an iNOS inhibitor, the yield of bystander MN was partly diminished, and the reducing rate of DMSO was 42.2%-46.7%, the reducing rate of aminoguanidine was 42%.Conclusion ROS, NO and their downstream signal factors are involved in the radiation induced bystander effect of hypoxic HepG2 cells. |
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