| JIN Xiao-dong,GONG Li,LI Qiang,et al.Survivin as a factor of radioresistance to high-LET carbon ions in human hepatoma SMMC-7721 cells[J].Chinese Journal of Radiological Medicine and Protection,2009,29(1):1-4 |
| Survivin as a factor of radioresistance to high-LET carbon ions in human hepatoma SMMC-7721 cells |
| Received:May 28, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.001 |
| KeyWords:SMMC-7721 cell Survivin Apoptosis RNA interference |
| FundProject:国家高技术研究发展计划(863计划)资助项目(2006AA02Z499);中国科学院“百人计划”资助项目(0506120BR0) |
| Author Name | Affiliation | E-mail | | JIN Xiao-dong | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | GONG Li | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | LI Qiang | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | liqiang@impcas.ac.cn | | HAO Ji-fang | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | LI Ping | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | WU Qing-feng | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | HE Jing | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | LIU Xin-guo | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | | | DAI Zhong-ying | Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China | |
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| Abstract:: |
| Objective To investigate the influences of survivin down-regulation on cell G2/M phase arrest,apoptosis and sensitivity to carbon ion irradiation.Methods Small interfering RNA (siRNA) targeting survivin mRNA was designed, in vitro chemo-synthesized and transfected into SMMC-7721 cells. Survivin mRNA expression in SMMC-7721 cells was measured by real-time PCR, and the apoptotic rates by Annexin-FITC at 24 and 48 h after transfection. Cell G2/M phase arrest after transfection was assessed with flow cytometry as well. Cellular sensitivity to high-LET carbon ions was determined by means of colony-forming assay.Results The expressions of survivin at mRNA level were down-regulated to be 59% and 39% in relation to the non-treated cells at 24 and 48 h after siRNA transfection, respectively. G2/M phase arrest in SMMC-7721 cells at 24 h after transfection was observed while much more obvious at 48 h. The apoptotic rate of SMMC-7721 cells was 21.41% at 48 h after survivin siRNA transfection, which was significantly higher than that of the cells transfected with negative siRNA. Moreover, a decreased clonogenic survival in siRNA treated group was shown.Conclusion Down-regulation of survivin gene expression in SMMC-7721 cells by siRNA could effectively induce cell apoptosis and G2/M phase arrest, and enhance the cellular radiosensitivity to high-LET heavy ions. |
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