| WEI Shu-zhen,XIE Xue-jun,QI Yu-hong.The hemoprotective effects of a rotary magnetic field in mice exposed to irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):660-664 |
| The hemoprotective effects of a rotary magnetic field in mice exposed to irradiation |
| Received:November 27, 2007 |
| DOI： |
| KeyWords:Rotary magnetic field Radiation injury Bone morphogenetic protein2/4 Hemoprotection |
| FundProject:第四军医大学军事预防医学系军事医学科研基金资助项目(XJ200604) |
| Author Name | Affiliation | E-mail | | WEI Shu-zhen | Department of radiation medicine, Faculty of Preventive Medicine, The Fourth military medical university, Xi'an 710032, China | | | XIE Xue-jun | Department of radiation medicine, Faculty of Preventive Medicine, The Fourth military medical university, Xi'an 710032, China | | | QI Yu-hong | Department of radiation medicine, Faculty of Preventive Medicine, The Fourth military medical university, Xi'an 710032, China | | | 郭国祯 | Department of radiation medicine, Faculty of Preventive Medicine, The Fourth military medical university, Xi'an 710032, China | guozhen@fmmu.edu.cn | | 山顺林 | 解放军第八二医院肿瘤诊疗中心 | | | 李前文 | 解放军第八二医院肿瘤诊疗中心 | | | 张闯 | 解放军第八二医院肿瘤诊疗中心 | | | 杜云翔 | 解放军第八二医院肿瘤诊疗中心 | |
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| Abstract:: |
| Objective To study the hemoprotective effects of a rotary magnetic field(RMF) to radiation-injured mice.Methods 132 male BALB/c mice were randomly divided into four groups: a normal group (N), a magnetic treatment group (M), an irradiation group(R) and an irradiation combining magnetic treatment group (R+M). Mice in the N group received no treatment. Mice in the R and R+M groups received total body irradiation with 6.0 Gy 60Co γ rays. Mice in the M and R+M groups were treated with a RMF for one and half an hour at a time,twice a day, totally for 30 days. The survival rate was observed for 30 days. On days 0, 5, 9, 15, 21, 30, the subjects peripheral blood cells were counted. On day 9, 23 and 30, the number of bone marrow nucleated cells (BMNCs), colony forming unit-spleen (CFU-S), spleen-body ratio, the cell cycle and apoptosis of bone marrow cells were measured. The pathological sectioning of the femur was performed and the expression level of bone morphogenetic proteins (BMP2/4) in the bone marrow was evaluated.Results 1No mice died in the N and M group. The RMF treatment increased the survival rate and survival days among the irradiated mice (P<0.01). 2The RMF treatment increased the number of blood cells in their peripheral blood of the R+M group. 3The number of BMNCs, CFU-S and the proportation of G2+M stage in the R+M were markedly higher than that of the R group, but the proportation of the apoptosis was lower than that of the R group on the 9th day (P<0.05). Furthermore, the spleen index in the R+M group was also higher than that of the R group on the 23rd day (P<0.05). 4RMF could improve the expression level of BMP2/4 in the radiation-injued mice.Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation and it accelerated the recovery of hematopoiesis and the hematopoietic microenvironment in mouse bone marrow. |
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