| HAN Chun-shan,LIANG Jun,LI Ling.Protective effect of polypeptides from Chlamys farreri on murine thymocytes damaged by 60Coγ-rays[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):618-620 |
| Protective effect of polypeptides from Chlamys farreri on murine thymocytes damaged by 60Coγ-rays |
| Received:November 29, 2007 |
| DOI： |
| KeyWords:Polypeptides from Chlamys farreri (PCF) 60Co γ-rays Lymphocyte apoptosis Murine thymocytes |
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| Abstract:: |
| Objective To study the effect and mechanism of polypeptide from Chlamys farreri(PCF) on the thymocytes damaged by 60Co γ-rays. Methods The cells were randomly divided into six groups: control group, 60Co groups (2Gy, model), 60Co +0.5% PCF, 60Co +0.25% PCF, 60Co +0.125% PCF, 60Co +0.1%VitC. The concentration of GSH-Px, ROS, A-SAC, T-AOC and the cells' viability were determined. The mitochondria membrane potential were tested. The expressions of P53, Bax and Bcl-2 proteins were examined.Results The activities of GSH-Px, A-SAC, T-AOC in cells were enhanced, and the amounts of ROS were decreased by PCF. The expression of Bcl-2 gene was up-regulated, and down-regulated for the expression of P53 and Bax. All observed indexes of the PCF groups were significantly different compared with model group (P<0.05).Conclusions PCF has the protective effects on damages of thymocytes caused by 60Co irradiation. The mechanisms might be related to inhibiting of lymphocyte apoptosis and enhancing the activities of lymphocytes. |
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