| REN Rui-mei,HUANG Guang-wu,SUN Xin-dong,et al.Radiation enhancement effect of RNA interference for HIF-1α on the transplant tumor[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):612-614 |
| Radiation enhancement effect of RNA interference for HIF-1α on the transplant tumor |
| Received:November 28, 2007 |
| DOI： |
| KeyWords:RNA interference HIF-1α Transplant tumor Radiosensitivity |
| FundProject:山东省科技厅博士基金资助项目(2006BS03035) |
| Author Name | Affiliation | | REN Rui-mei | Department of Radiation Oncology, Shandong Cancer Hospital & Institute, Jinan 250117, China | | HUANG Guang-wu | 广西医科大学 | | SUN Xin-dong | Department of Radiation Oncology, Shandong Cancer Hospital & Institute, Jinan 250117, China | | 赵汉玺 | Department of Radiation Oncology, Shandong Cancer Hospital & Institute, Jinan 250117, China | | 闫庆霞 | Department of Radiation Oncology, Shandong Cancer Hospital & Institute, Jinan 250117, China |
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| Abstract:: |
| Objective To determine and explore the radiation enhancement of RNA interference for HIF-1α on the transplant tumor using polycationic polyethyleninine (PEI), as a new kind of gene vector. Methods SPCA-1 nude mouse model was used. 160 nude mice bearing SPCA-1 were randomly divided into 4 treated groups and 1 control groups, each group had 32 mice. The expression of HIF-1α was studied by immunohistochemical method after RNA interference for HIF-1α. The differences of the volume, weight, survival time of the transplant tumor were studied among the simple radiation group, the simple RNA interference for HIF-1α group and the combination of radiation and RNA interference for HIF-1α. Results The expression of HIF-1α was decreased after RNA interference for HIF-1α. RNA interference for HIF-1α combined with radiation decreased the volume, weight of the transplant tumor, and prolonged its survival time period significantly than other methods.Conclusions RNA interference targeting HIF-1α might enhance the radiosensitivity of the transplant tumor using PEI as a new kind of gene vector in vitro. |
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