| XIAO Mang,TANG Jian-guo,SHI Guo-zhi.Early remodeling of nasal mucosa in rat model after radiation injury[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):602-605 |
| Early remodeling of nasal mucosa in rat model after radiation injury |
| Received:November 12, 2007 |
| DOI： |
| KeyWords:Radiation injury Animal models Epithelium Goblet cell Extracellular matrix |
| FundProject:浙江省自然科学基金资助项目(Y205073) |
| Author Name | Affiliation | E-mail | | XIAO Mang | Department of Otorhinolaryngology-Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou 310016, China | | | TANG Jian-guo | Department of Otorhinolaryngology-Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou 310016, China | zhengzheng@mail.hz.zj.cn | | SHI Guo-zhi | 310016 杭州, 浙江大学医学院附属邵逸夫医院耳鼻咽喉-放疗科浙江省生物治疗重点实验室 | | | 罗宝珍 | Department of Otorhinolaryngology-Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou 310016, China | | | 赵丽娜 | Department of Otorhinolaryngology-Head and Neck Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou 310016, China | |
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| Abstract:: |
| Objective To explore the feature of nasal mucosa remodeling in experimental radiation injury.Methods Fourty male rats were randomly divided into five groups, as control group and radiation injury groups (radiation dose were 20Gy,30Gy,40Gy and 50Gy). Each group had 8 rats. Two weeks after the last irradiation, the rats were killed and the nasal middle turbinates of the animals were removed. The tissue blocks were embedded in paraffin. The paraffin sections were stained with hematoxylin and eosin (HE),alcian blue-periodic acid-Schif (AB-PAS),and Masson Trichrome (MT).The infiltrating eosinophils in nasal mucosa were examined.AB-PAS positive cells in the surface epithelium in nasal mucosa were counted.The percentage of area in MT stained extracellular matrix in nasal mucosa and damage of epithelium were determined by an image analyzer.Results The control group only presented a few eosinophils.Significant eosinophil infiltration was observed in the radiation injury groups, especially for the 30Gy radiation injury group. Compared with the control group, there was no significant epithelial damage in 20Gy radiation injury group.Significant epithelial damage were observed in the rest of radiation injury groups.The epithelial damage became more severe as the radiation dose increasing. A little but not significant increase in AB-PAS positive cells was observed in the mucos of the 20Gy radiation injury group and significant increase in the 30 and 40 Gy groups.But in the 50 Gy radiation injury group, the AB-PAS positive cells were decreased compared with control group. The collagen fibrils in the mucosa of nasal middle turbinate in 20 Gy radiation injury group did not significantly increase. But in the other groups,the increase was significant compared with that of control group. Furthermore,collagen fibrils increased as the radiation dose increased.Conclusions Epithelial damage, goblet cells hyperplasia and extracellular matrix deposition are the features of remodeling in the rats model of early radiation injury. |
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