| MA Shu-mei,WU Li-min,LIU Yang.Roles of p53 in ionizing radiation-induced cell cycle uncoupling[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):597-600 |
| Roles of p53 in ionizing radiation-induced cell cycle uncoupling |
| Received:November 15, 2007 |
| DOI： |
| KeyWords:p53 gene Cell cycle uncoupling RNAi Ionizing radiation |
| FundProject:国家自然科学基金资助项目(30500142、30770649);吉林省科技发展计划资助项目(2005136);教育部留学回国人员科研启动资助项目(2005) |
| Author Name | Affiliation | E-mail | | MA Shu-mei | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | WU Li-min | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | LIU Yang | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 施丹 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 易贺庆 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 张洁琼 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 汪海娇 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 刘晓冬 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | liuxd2002@yahoo.com |
|
| Hits: 2719 |
| Download times: 2049 |
| Abstract:: |
| Objective To explore the roles of p53 in ionizing radiation induced MCF-7 cell cycle uncoupling.Methods The p53 knock-down models was established in MCF-7 with retrovirus packaged particles from 293T cells through calcium acid phosphate co-precipitation, then Western blot was used to detect the protein expression. Flow cytometry(FCM) was used to analyze the cell cycle uncoupling and polyploid after irradiation.Results Compared with p53+ + group, the percentages of G0 G1 cells in p53- - group decreased, while those of S and G2+M increased (P<0.01). In polyploidy analysis 2N cells decreased, whereas both 4N and 8N cells increased (P<0.01). Compared with sham-irradiation, 4 Gy X-ray led to the decrease of G0 G1, S cells, and the increase of G2+M cells. The increase of 2N cells and decrease of 4N and 8N cells were observed in both p53+ + and p53- - cells. Compared with p53+ ++IR group, the decrease of G0 G1 and S cells and the increase of G2+M cells were significant (P<0.01) in p53- -+IR groups. 2N cells decreased, 4N cells increased, but no changes in 8 N cells occurred. Conclusion Radiation might induce G2 arrest and cycle uncoupling. p53 plays a role in the regulation of G2 arrest, but no role in cycle uncoupling. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
