| LI Ming,SONG Liang-wen,WANG Shao-xia.Antagonist effect of Interferon-γ aerosol inhalation on pulmonary remodeling after γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):593-596 |
| Antagonist effect of Interferon-γ aerosol inhalation on pulmonary remodeling after γ-ray irradiation |
| Received:November 29, 2007 |
| DOI： |
| KeyWords:Interferon-γ Radiation pulmonary injury Matrix metalloproteinases Type Ⅳ Collagen |
| FundProject:国家自然科学基金资助项目(30570545) |
| Author Name | Affiliation | E-mail | | LI Ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | LSong1@yahoo.com.cn | | WANG Shao-xia | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 刁瑞英 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 徐新萍 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 罗庆良 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To observe the antagonistic effect of interferon-γ aerosol inhalation on pulmonary remodeling after γ-ray irradiation, and explore its mechanisms. Methods The Wistar rats were randomly divided into irradiation control group and irradiation + Interferon-γ antagonist group, which proceeded IFN-γ aerosol inhalation 3 days before 20 Gy 60Co γ-ray irradiation, then were sacrificed at 10, 20, 30 days after irradiation. Conventional histopathological sections of lung tissue were prepared, which were stained immunohistochemically for α-SMA and Sirius red. The contents of collagen Ⅳ were determined by Western blot. The expression of MMP-2, MMP-9 and TIMP-1 in lung homogenate was detected by ELISA. Results The widen degrees of interalveolar septum, the deposition of collagen Ⅰ, Ⅲ, and the expression of α-SMA decreased significantly in IFN-γ treatment group as compared with those in the irradiation control group. The expression of collagen Ⅳ appeared an elevation trend, but this phenomenon attenuated after IFN-γ was used. The levels of MMP-2 and TIMP-1 decreased 10 days after administration with IFN-γ, but the opposite trend appeared for MMP-9. The expression of MMP-2, MMP-9 and TIMP-1 decreased 30 days after administration with IFN-γ. Conclusion IFN-γ is effective in alleviating pulmonary injuries induced by irradiation in rats, possibly by decreasing the expression of TIMP-1 to relieve the inhibition to MMP-9, then degrading collagen Ⅳ to antagonize remodeling after lung injury. |
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