| SHAO Chun-lin,CHEN Hong-hong,MIZUHO Aoki.Influence of gap junctional intercellular communication on heavy ion irradiation induced cell responses[J].Chinese Journal of Radiological Medicine and Protection,2008,28(6):589-592 |
| Influence of gap junctional intercellular communication on heavy ion irradiation induced cell responses |
| Received:November 28, 2007 |
| DOI： |
| KeyWords:Heavy ion irradiation Neoplastic cell Primary human fibroblast GJIC Signaling factors |
| FundProject:国家自然科学基金资助项目(30670629,30770644);日本放射线医学综合研究所基金资助项目(13B330);教育部新世纪人才计划基金资助项目(NCET060365);上海市浦江人才计划基金资助项目(06PJ14012) |
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| Abstract:: |
| Objective To investigate the influence of GJIC on heavy-ion irradiation induced cellular damage and associated mechanism. Methods Primary human fibroblast AG1522 and human neoplastic HSG cells under confluence were irradiated with high-energy carbon beam. The influence of gap junctional intercellular communication (GJIC) on radiation responses of micronucleus (MN) formation and cycle arrest was measured. Results Cell lethal damage and cell cycle arrest were induced by high energy carbon beam, and AG1522 cells had radiosensitivity higher than HSG cells. For irradiated AG1522, cellular damage was positively adjusted by GJIC. However, with respect to irradiated HSG cells, cell damage was diminished by enhanced GJIC. Moreover, MN in AG1522 was reduced by DMSO and MN in HSG cells was increased by PTIO. Conclusions GJIC increases radiation damage on primary fibroblasts but has radiation protective effect on tumor cells. ROS and NO may contribute to these GJIC mediated radiation responses on AG1522 and HSG cells, respectively. |
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