| WEI Li,PENG Rui-yun,GAO Ya-bing.Changes in expressions of PSD-95 and CREB in hippocampi of rats after exposure to high power microwave[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):545-549 |
| Changes in expressions of PSD-95 and CREB in hippocampi of rats after exposure to high power microwave |
| Received:August 21, 2007 |
| DOI： |
| KeyWords:High power microwave Hippocampus Postsynaptic density-95 Cyclic AMP-response element binding protein |
| FundProject: |
| Author Name | Affiliation | E-mail | | WEI Li | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | pengry@nic.bmi.ac.cn | | GAO Ya-bing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王水明 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 马俊杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 徐新萍 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 苏镇涛 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 杨国山 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate the change of the expressions of PSD-95 and CREB in hippocampi of rats after exposure to high power microwave (HPM). Methods The HPM device with mean power densities of 10, 30 and 100 mW/cm2 was used to expose 75 healthy Wistar male rats, which were killed on at hour 6, days 1, 7, 14 and 28 after irradiation. The expressions of PSD-95 and CREB were analyzed by immunohistochemistry, gel retardation assay and imaging analysis. Results The expression of PSD-95 in hippocampi of rats was up-regulated after 10 and 100 mW/cm2 exposure. The levels of PSD-95 in hippocampi rised at hour 6 after exposure to 10 mW/cm2, peaked at day 1, and returned to the basal level at day 28 after exposure. The levels of PSD-95 went up at hour 6 after 100 mW/cm2 exposure, peaked at day 1 (MOD) and 7 (IOD) respectively, and returned to the basal level at day 28 after 100 mW/cm2
exposure. The binding ability of CREB and DNA was declined within 3 d after exposure to 30 mW/cm2 HPM. Conclusions The levels of PSD-95 in hippocampi are increased, but the binding ability of CREB and DNA declined after exposure to HPM, which is contributed to the patho-physiological process of hippocampus tissue injury. |
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