| LONG Qing-yun,ZHOU Yun-feng,HU Jin-xiang.Effect of Angelica Sinensis on liver fibrosis after radiotherapy combined with transarterial chemoembolization for rabbits with VX 2 tumor[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):514-516,538 |
| Effect of Angelica Sinensis on liver fibrosis after radiotherapy combined with transarterial chemoembolization for rabbits with VX 2 tumor |
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| DOI： |
| KeyWords:Rabbit Hepatic fibrosis Chemoembolization Radiotherapy Angelica Sinensis injection |
| FundProject: |
| Author Name | Affiliation | E-mail | | LONG Qing-yun | Department of Radiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | ZHOU Yun-feng | Department of Radiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China | yfzhouwhu@163.com | | HU Jin-xiang | Department of Radiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | 周军 | Department of Radiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China | | | 乐涛 | Department of Radiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China | |
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| Abstract:: |
| Objective To evaluate the effect of Angelica Sinensis on liver fibrosis after radiotherapy(RT) combined with transarterial chemoembolization(TACE) for rabbits with VX 2 tumor, and the mechanism of anti-hepatic fibrosis. Methods Fifty New Zealand white rabbits were divided randomly into three groups:Control group(n=10) was given saline 2 ml by gastroduodenal artery, model group (n=10) was given pingyangmycin 1 mg and iodized oil 0.2 ml, and exposed to single fraction of 20 Gy, Angelica group(n=30) undergoing RT combined with TACE was given Angelica Sinensis injection. According to different doses of Angelica Sinensis, Angelica group was divided into low-dose group (4 ml/kg), middle dose group (6 ml/kg) and high-dose group (8 ml/kg), twice per week for 4 weeks.Serum levels of HA, LN, PCⅢ, CIV and liver specimens were obtained at the end of 6th week after RT combined with TACE.The histological changes were determined by HE,VG staining and TGF-β1 immunohistochemistry staining. Results Stages of liver fibrosis in the model group were mostly stages Ⅱ and Ⅲ, and stages of liver fibrosis in the Angelica group were mostly stages I,there was significant difference between the model group and the Angelica group, and stages of liver fibrosis were associated with Angelica intervention treatment (r=0.7631, P<0.01). Serum HA, LN, PCⅢ and CIV in the model group were higher than those in the control group(P<0.01), TGF-β1 expression in the Angelica group was significantly reduced compared with the wodel group and different doses of Angelica group showed dose-effect (r=0.4427, P<0.01). Conclusions Angelica Sinensis injection has inhibitive effect on liver fibrosis after RT combined with TACE for rabbits with VX 2 tumor,the mechanism of anti-hepatic fibrosis might possibly be associated with down-regulating TGF-β1. |
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