| ZHANG Li-yuan,HUO Hong-mei,ZHANG Yu-song,et al.Low dose radiation induced hormesis and its mechanism of free radicals[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):511-513 |
| Low dose radiation induced hormesis and its mechanism of free radicals |
| Received:August 13, 2007 |
| DOI： |
| KeyWords:Low dose radiation Hormesis Free radicals |
| FundProject:苏州市科教兴卫基金资助项目(SWQ19) |
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| Abstract:: |
| Objective To investigate whether the supernatant (the stimulating fluid) centrifuged from myeloid cells suspension after low dose radiation in vitro can produce hormesis on the normal or radiation damage cells. The mechanism of free radical was probed. Methods Mouse myeloid cell suspension was irradiated respectively by 0, 2 and 5Gy, and cultured in vitro. MTT method was used to measure the reproductive activity of cells. Meanwhile, Cytochrome C reduction method was used to determine the concentration of O2-. Lastly, the concentration of O2- was decreased or increased by adding DPI or PMA, and the effect of such changes on "the stimulating fluid" was observed. Results Co-cultured with "the stimulating fluid", the reproductive activity of the myeloid cells after large dose radiation or the normal myeloid cells were enhanced. Decreasing the concentration of O2- may degrade the proliferation of the cells after radiation damage; while increasing it may lead to the opposite result. Conclusions The stimulating fluid can enhance the proliferation of the myeloid cells after radiation damage and also the normal ones. The mechanism of above-mentioned phenomena might be related with the changes of O2- concentration. |
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