| ZHOU Ju-ying,TU Yu,et al.Radiosensitization of acetaminophen on human glioma cell lines and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):502-505 |
| Radiosensitization of acetaminophen on human glioma cell lines and its mechanism |
| Received:June 27, 2007 |
| DOI： |
| KeyWords:Radiosensitization COX-2 Acetaminophen Glioma |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHOU Ju-ying | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | TU Yu | 苏州大学放射医学与公共卫生学院 | tuyu@suda.edu.cn | | 徐晓婷 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | 俞志英 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | 秦颂兵 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | 王利利 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | 李莉 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | | | 周乐源 | Department of Radiation Oncology, First Affiliated Hospital to Suzhou University, Suzhou 215006, China | |
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| Abstract:: |
| Objective To investigate the radiosensitivity enhancement and underlying mechanism of acetaminophen ,non-selective cyclooxygenase (COX)-2 inhibitor, on human glioma cell lines expressing differential COX-2 levels. Methods The SHG-44 cells were irradiated with a dose of 10 Gy using 6MV X-rays generated by linar accelerator. The progeny of the cells were cultured and named SHG-4410Gy. COX-2 mRNA and protein expression of SHG-44 and SHG-4410Gy were detected by RT-PCR and immunocytochemisty staining. Clongenic assay was used for radiation survival experiment. Results The declined radiosensitivity was detected in the SHG-4410Gy. RT-PCR showed that the expression of COX-2 mRNA in SHG-4410Gy significantly higher than that in SHG-44 cells (P<0.01). The cell inhibition induced by Acetaminophen and irradiation was positively correlated with the expression of COX-2 mRNA(P<0.01). was observed from the dose-survival curve and the related parameters. The values of SER were 1.09(D0) or 1.11(Dq)in SHG-44 cells and 1.12(D0)or 3.01(Dq)in SHG-4410Gy. Conclusions SHG-4410Gy cells are more radio-resistant, and one of the fundamental mechanisms might be the upregulation of COX-2 expression in protein and mRNA levels. Acetaminophen could enhance the radisensitivity of glioma cells, especially the surviving progeny from the irradiated SHG-44 cells. |
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