| JIANG Ding-wen,LEI Cheng-xiang,SHEN Xian-rong.Effects of low dose rate fission neutron irradiation on the lymphocyte subpopulations of peripheral blood in rats\[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):488-491 |
| Effects of low dose rate fission neutron irradiation on the lymphocyte subpopulations of peripheral blood in rats\ |
| Received:August 29, 2007 |
| DOI： |
| KeyWords:Fission neutron Low dose rate Lymphocyte subpopulations Rat |
| FundProject: |
| Author Name | Affiliation | E-mail | | JIANG Ding-wen | Naval Medical Research Institute, Shanghai 200433, China | | | LEI Cheng-xiang | Naval Medical Research Institute, Shanghai 200433, China | cxlei@yeah.net | | SHEN Xian-rong | Naval Medical Research Institute, Shanghai 200433, China | | | 马丽 | Naval Medical Research Institute, Shanghai 200433, China | | | 杨翊方 | Naval Medical Research Institute, Shanghai 200433, China | | | 彭武林 | Naval Medical Research Institute, Shanghai 200433, China | | | 戴寿荣 | Naval Medical Research Institute, Shanghai 200433, China | |
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| Abstract:: |
| Objective To evaluate the effects of long-term, low dose rate fission neutron irradiation on lymphocyte subpopulations in peripheral blood of rats. Methods Ninety-six rats were randomly divided into control group and irradiated group exposed to low dose rate fission neutron (252Cf,0.35 mGy/h) for 20.5 h every day.At days 14,28,42,56 and 70 d after irradiation and 35 d after stopping irradiation, After 8 rats of each group were killed, WBC and lymphocyte subpopulations of CD4+CD3+,CD8+CD3+and CD45RA+/CD161α+ in peripheral blood were estimated respectively. Results Compared with the control group,WBC was reduced significantly at dose of 0.3, 0.4 and 0.5Gy (P<0.05), and was decreased remarkably at 35 d after stopping irradiation(P<0.01). The rate of CD4+CD3- was evidently higher compared with control group at doses of 0.1,0.3, 0.4 and 0.5 Gy and 35 d after stopping irradiation (P<0.01, P<0.01 and P<0.05), and the rate of CD8+CD3- was obviously higher compared with control group at dose of 0.2 and 0.3 Gy (P<0.05 and P<0.01). On the other hand, the rate of CD4+CD3+ at dose of 0.1 Gy (P<0.01) and the rate of CD8+CD3+ at doses of 0.1 and 0.2 Gy (P<0.05) were higher when compared with control group. At the same time,peripheral blood NK cells(CD161α+ CD45RA-) was increased significantly at doses of 0.2-0.3 Gy, and peripheral blood B cells(CD161α- CD45RA+) was reduced remarkably at doses of 0.1-0.5 Gy and 35 d after stopping irradiation compared with the control group. Conclusions Long-term irradiation with low dose rate fission neutron could make TCR (T-cell-receptor) mutant, therefore, WBC, B cells in peripheral blood significantly reduced and NK cells increased. These changes may could not recover at 35 d after stopping irradiation. |
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