| WANG Peng,ZHANG Xiang,JIANG Xin-biao.Boron neutron capture therapy induced apoptosis in SHG44 glioma cells[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):484-487 |
| Boron neutron capture therapy induced apoptosis in SHG44 glioma cells |
| Received:October 25, 2007 |
| DOI： |
| KeyWords:Boron neutron capture therapy(BNCT) SHG44 Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | WANG Peng | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | | | ZHANG Xiang | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | xzhang@fmmu.edu.cn | | JIANG Xin-biao | 西北核技术研究所 | | | 程光 | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | | | 赵英 | 西北核技术研究所 | | | 甄海宁 | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | | | 王剑博 | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | | | 曹锐峰 | Neurosurgery Institute of Chinese PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China | |
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| Abstract:: |
| Objective To investigate the inhibition of boron neutron capture therapy(BNCT) on SHG44 glioma cell line and its mechanism. Methods Methyl thiazolyl tetrazolium(MTT) assay was used to measure the level of the proliferation of SHG44. HE staining, Hoechst33342 fluorescence staining, transmission electron microscope(TEM) were applied to observe the changes in cell morphology. The effects of BNCT on cell apoptotic rate was observed by flow cytometer (FCM). The expression of Bcl-2 and Bax protein was measured by Western blot. Results The proliferation of SHG44 was obviously inhibited by BNCT in a dose-dependent manner. The results of FCM showed that the apoptotic cell rate 48 h after irradiation with 4 and 8 Gy 63.2% and 88.3%, respectively. Western blot analysis showed BNCT promoted Bax protein expression,but inhibited Bcl-2 expression. Conclusions BNCT inhibits the proliferation of SHG44 cell, induces apoptosis and promote the expression of Bax but inhibits the expression of Bcl-2. |
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