| LI Ming,SONG Liang-wen,DIAO Rui-ying,et al.Role of MMP-12 on tissue remodeling at early stage of radiation_induced pulmonary injury[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):479-483 |
| Role of MMP-12 on tissue remodeling at early stage of radiation_induced pulmonary injury |
| Received:May 08, 2008 |
| DOI： |
| KeyWords:Macrophage metalloproteinase-12 Radiation pulmonary injury Elastin Cross talking Tissue remodeling |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | LSong1@yahoo.com.cn | | DIAO Rui-ying | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王少霞 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 徐新萍 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 罗庆良 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the role of MMP-12 on tissue remodeling at early stage of radiation_induced pulmonary injury. Methods Wistar rats irradiated by 60Co γ-rays to the whole lungs were sacrificed at 1, 2, 4 weeks. MMP-12 mRNA expression was detected by RT-PCR. MMP-2, MMP-9, MMP-12 activities were determined by zymography.The degradation and collapse of elastin were determined by tissue elastin particular staining; the "cross talking" phenomenon between alveolar typeⅡ cells and mesenchymal cells was observed under electron microscope; the expression of TGF-β1 and TNF-α in BALF was detected by ELISA. The expression of α-SMA was determined by immunohistochemistry. Results The mRNA expression of MMP-12 displayed a significant elevation at 1, 2, 4 weeks after irradiation. MMP-12 activity increased at 2, 4 weeks after irradiation. Elastin began to degrade and collapse at 1 week, which became worst 4 weeks after irradiation. The cross talking phenomenon was found under electron microscope. The expression of TGF-β1, TNF-α and α-SMA was increased gradually as time elapse after irradiation. Conclusions 60Co γ-ray irradiation can promote pulmonary MMP-12 expression, initiate pulmonary tissue remodeling by degradation of elastin, and make the pulmonary injury develop towards pulmonary fibrosis eventually. |
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