| GAO Fu,CAI Jian-ming,CUI Jian-guo,et al.Alterations of INK4A gene and P16 protein in leukemia mice model induced by 60Co γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):475-478 |
| Alterations of INK4A gene and P16 protein in leukemia mice model induced by 60Co γ-ray irradiation |
| Received:September 17, 2007 |
| DOI： |
| KeyWords:INK4A Leukemia model Irradiation P16 protein |
| FundProject: |
| Author Name | Affiliation | E-mail | | GAO Fu | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | CAI Jian-ming | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | cjm882003@yahoo.com.cn | | CUI Jian-guo | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 杨平 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 黄越承 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 李百龙 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 闵锐 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 倪瑾 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | | | 孙顶 | Department of Radiomedicine, Second Military Medical University, Shanghai 200433, China | |
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| Abstract:: |
| Objective To investigate the alterations of INK4A gene and protein in the leukemia model of mice induced by 60Co γ-ray irradiation. Methods BALB/c mice were irradiated 4 times weekly with 60Co γ-ray irradiation to establish the animal model of lymphocyte leukemia, and the total absorbed dose was 7.0 Gy. PCR was used to amplify exons 1 and 2 of INK4A gene and to detect the homozygous deletion. The occurrence of methylation in the 5'CpG island was studied with methylation sensitive enzyme PCR. PCR-SSCP-silver staining technique was used to detect the mutation. The expression of P16 protein was detected with Western blot. Results In 21 cases of leukemia model tissues, there were 5 cases of depletion of the exon 2 of INK4A gene, and 4 cases of methylation in the priming domain of the exon 1, 11 cases of protein expression decreased. Conclusions The decrease in p16 expression might participate in the radiation- induced tumorigenesis. The homozygous deletion and methylation are the main causes of inactivation of INK4A gene in mice. |
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