| WU Ning,SHAN Yu-xing,JIN Shun-zi.Anti-tumor immunological mechanisms of low dose whole-body irradiation in the protocol of tumor gene-radiotherapy[J].Chinese Journal of Radiological Medicine and Protection,2008,28(5):441-444 |
| Anti-tumor immunological mechanisms of low dose whole-body irradiation in the protocol of tumor gene-radiotherapy |
| Received:May 08, 2008 |
| DOI： |
| KeyWords:Low dose irradiation Tumor gene-radiotherapy Lewis lung carcinoma |
| FundProject: |
| Author Name | Affiliation | E-mail | | WU Ning | Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | | | SHAN Yu-xing | 吉林大学第一临床医学院骨科 | | | JIN Shun-zi | Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | shunzij@yahoo.com.cn | | 刘树铮 | Key Laboratory of Radiobiology, Ministry of Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To investigate the immunologic enhancement of low dose whole-body irradiation in mice bearing Lewis lung carcinoma (LLC) under recombinant plasmid pEgr-IL18-B7.1 gene-radiotherapy. Methods LLC cells were implanted subcutaneously in the right-hind leg of C57BL/6J mice. The pEgr-IL18-B7.1 recombinant plasmids mediated by polyethylenimine were injected locally into tumors of the mice with gene-radiotherapy,and then the tumors received different therapeutic regimens containing local irradiation with 2 Gy and whole-body irradiation with 0.075 Gy, respectively. Cytotoxic activity of CTL and NK were detected with isotope labeling of 3H-TdR. The secretion activities of TNF-α and IFN-γ were detected with ELISA. The anti-tumor immunological effects of low dose whole-body irradiation in protocol of gene-radiotherapy on the tumor-bearing mice were observed. Results Compared with conventional repeated high dose local irradiation, single high dose local irradiation in combination with repeated low dose whole-body irradiation could enhance the cytotoxic activity of CTL and NK,and increase the secretion of TNF-α and IFN-γ under pEgr-IL18-B7.1 gene-radiotherapy. Conclusions Low dose whole-body irradiation superimposed upon a local high dose could significantly enhance the anti-tumor effect in the protocol of gene-radiotherapy through promoting the cytotoxic activity of CTL and NK,and up-regulating the expression of TNF-α and IFN-γ. |
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