| ZHAO Li,PENG Rui-yun,GAO Ya-bing,et al.Effects of fructose-1,6-diphosphate on COX expression of mitochondrial respiratory chain in rats hippocampus after microwave exposure[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):421-423 |
| Effects of fructose-1,6-diphosphate on COX expression of mitochondrial respiratory chain in rats hippocampus after microwave exposure |
| Received:February 28, 2008 |
| DOI： |
| KeyWords:FDP Microwave Rat Hippocampus Mitochondria COX |
| FundProject:军队医药卫生“十一五”专项基金资助项目(06Z065) |
| Author Name | Affiliation | E-mail | | ZHAO Li | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | PENG Rui-yun | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | pengry@nic.bmi.ac.cn | | GAO Ya-bing | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王水明 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王晓民 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 徐新萍 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王丽峰 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 董霁 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 杨国山 | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate the effects of fructose-1,6-diphosphate (FDP) on the gene COX expression of mitochondrial respiratory chain in rats hippocampus after microwave exposure.Methods 25 male rats were exposed to microwave that the average power density was 30 mW/cm2. FDP was injected intraperitoneally once per day 3 d before exposure, lasting 3ds or 10ds. Rats were sacrificed at 6h and 7d after exposure. Reverse transcription-polymerase chain reaction (RT-PCR) and image analysis were used to study the expression of COX Ⅰ, Ⅱ and ⅣmRNA. The COX Ⅰ protein was detected by Western blot and image analysis.Results The expression level of COX Ⅰ, Ⅱ and Ⅳ mRNA were decreased at 6h and 7d after exposure while in administration group the expression level increased especially at 3d after administration (tCOX Ⅰ=3.2205, tCOX Ⅱ=3.5762, tCOX Ⅳ=3.0408, P<0.05). The COX Ⅰ protein level decreased after exposure while it increased at 3 d after administration (t =2.9614, P<0.05), but there was no significant change at 10d after administration.Conclusions FDP could recover the decrease of COX expression level of mitochondrial respiratory chain in rats hippocampus after microwave exposure. |
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