| CHEN Xue,LIU Li,DONG Ji-hua,et al.Modulation role of Shenqi Fuzheng Injection on transforming growth factor beta 1 (TGF-β1) expression induced by radiation in human lung adenocarcinoma GLC-82 cells[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):358-360 |
| Modulation role of Shenqi Fuzheng Injection on transforming growth factor beta 1 (TGF-β1) expression induced by radiation in human lung adenocarcinoma GLC-82 cells |
| Received:January 08, 2008 |
| DOI： |
| KeyWords:TGF-β1 Radiation-induced injury Shenqi Fuzheng Injection Human lung adenocarcinoma GLC-82 |
| FundProject:中国临床肿瘤学科学基金资助项目(Y20050040);湖北省科技厅科技公关项目(2005AA401C25) |
| Author Name | Affiliation | E-mail | | CHEN Xue | Tumor Center, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430023, China | | | LIU Li | Tumor Center, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430023, China | liulixiehe2004@163.com | | DONG Ji-hua | 430023 武汉, 华中科技大学附属协和医院中心实验室 | | | 丁乾 | Tumor Center, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430023, China | |
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| Abstract:: |
| Objective To investigate the ability of Shenqi Fuzheng Injection on release of TGF-β1 mRNA in human lung adenocarcinoma GLC- 82 cells by ionizing radiation in vitro,and find the regulation mechanism to reduce the radiation-induced lung injury by traditional Chinese drug.Methods GLC-82 cells were cultured in media with serum containing 1.563 mg/ml Shenqi Fuzheng Injection,then TGF-β1 mRNA after exposure to different irradiation doses(0, 20,30,40 Gy) and time intervals(12,24,48 h) were investigated. The expression of TGF-β1 mRNA detected semi-quantitatively by RT-PCR.Results For the different irradiation doses group, TGF-β1 expression were increased after irradiation, which was significantly elevated at 20 Gy compared with unirradiated cells. The GLC-82 cells,treated by Shenqi Fuzheng Injection, revealed a minor radiation mediated TGF-β1 response on mRNA level, which was significantly different at 20 Gy compared with the irradiated group (P<0.05). For the different time intervals group, following irradiation exposure in 20 Gy, the TGF-β1 mRNA levels in cells, which were treated by Shenqi Fuzheng Injection, were down-regulated and significantly decrease at 48 h (P<0.01).Conclusions The transforming growth factor beta 1 -TGF-β1 is a key mediator for the pathogenesis of radiation-induced lung injury. Shenqi Fuzheng Injection down-regulates the radiation- induced TGF-β1 release, and it might reduce the lung toxicity after thoracic irradiation. |
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