| QIN Jian,WANG Ren-sheng,TENG Jia-an,et al.Experimental study on the effect of Sarcandra glabra extracts on radiation-induced acute injury to parotid gland[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):351-353 |
| Experimental study on the effect of Sarcandra glabra extracts on radiation-induced acute injury to parotid gland |
| Received:August 26, 2007 |
| DOI： |
| KeyWords:Sarcandra glabra Hydroxy free radical Parotid gland Radiation injury |
| FundProject:广西壮族自治区自然科学基金资助项目(桂科自0542090) |
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| Abstract:: |
| Objective To explore the effect of Sarcandra glabra extracts on hydroxy free radical (OH·), and acute radiation injury to guinea pig's parotid gland.Methods Spectrophotometry was used and 1,10-Phenanthroline-Fe2+ hydrocarbonylation was applied to observe the scavenging effect of Sarcandra glabra extracts on OH· in vitro. 48 male guinea pigs were randomly divided into four groups as control group, radiation group,medicine group which received Sarcandra glabra extracts but no radiation, medicine+radiation group which received extracts and radiation. The radiation group and medicine+radiation group were received a single dose of 15 Gy gamma-rays delivered to head and neck region. 24 hours after radiation parotid gland tissues were obtained, and total activities of superoxide dismutase(SOD) and malonaldehyde(MDA) content were measured.Results At the Sarcandra glabra extracts concentrations of 0.2, 0.4, 0.6 and 1.2 mg/ml in vitro, the scavenging rate of OH· were 0.89％, 17.52％, 49.35％ and 89.89％, respectively. An obvious dose-effect relationship was observed with the concentration increased. MDA content of parotid gland were 2.99, 4.34, 0.74 and 1.3 nmol/mg of protein in control group, radiation group, medicine group and medicine+radiation group, respectively. There was a statistical significance among them(F=101.93,P=0.00). Total SOD activities of parotid gland were 249.6, 223.02, 262.23 and 165.05 U/mg of protein, respectively. There was no significant difference among control group, radiation group and medicine group, while medicine+radiation group was significantly lower than those of the above 3 groups. No linear relationship was observed between SOD and MDA(R=0.07).Conclusions Sarcandra glabra extracts could greatly scavenge OH· in vitro. It has a potential radioprotective effect against gamma-radiation-induced oxidative stress and acute injury to parotid gland. It might have an inhibitory action on total SOD activities of parotid gland after radiation. |
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