| MEI Que-lin,YANG Jian-yong,LI Yan-hao,et al.ATM phosphorylation in HepG2 cells following continuous low dose-rate irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):348-350 |
| ATM phosphorylation in HepG2 cells following continuous low dose-rate irradiation |
| Received:February 05, 2007 |
| DOI： |
| KeyWords:γ-irradiation HepG2 cells Proliferation Ataxia-telangiectasia mutated |
| FundProject:吴阶平医学科研基金资助项(320.6720.0501);深圳市科技计划项目(200404093) |
| Author Name | Affiliation | E-mail | | MEI Que-lin | Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China | | | YANG Jian-yong | 中山大学第一附属医院 | | | LI Yan-hao | 南方医科大学南方医院 | | | 杜端明 | Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China | | | 陈在中 | Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China | | | 刘鹏程 | Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China | liupeng-cheng66@163.com |
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| Abstract:: |
| Objective To investigate the change of ATM phosphorylation in HepG2 cells following a continuous low dose-rate irradiation. Methods Cells were persistently exposed to low dose-rate (8.28 cGy/h) irradiation. Indirect immunofluorescence and Western blot were used to detect the expression of ATM phosphorylated proteins. Colony forming assay was used to observe the effect of a low dose-rate irradiation on HepG2 cell survival. Results After 30 min of low dose-rate irradiation, the phosphorylation of ATM occurred. After 6 h persistent irradiation, the expression of ATM phosphorylated protein reached the peak value, then gradually decreased. After ATM phosphorylation was inhibited with Wortmannin, the surviving fraction of HepG2 cells was lower than that of the irradiation alone group at each time point (P＜0.05).Conclusions Continuous low dose-rate irradiation attenuated ATM phosphorylation, suggesting that continuous low dose-rate irradiation has a potential effect for increasing the radiosensitivity of HepG2 cells. |
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