| DING Wei-jun,YANG Hai-hua,WANG Xu-feng.1H-MR Spectroscopy of the rat hippocampus after whole brain irradiation: an in vivo study[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):343-347 |
| 1H-MR Spectroscopy of the rat hippocampus after whole brain irradiation: an in vivo study |
| Received:January 26, 2008 |
| DOI： |
| KeyWords:Magnetic resonance spectroscopy Radiation brain lesions Rat Microscopy Electron Cognition |
| FundProject:浙江省卫生厅资助项目(2005A109) |
| Author Name | Affiliation | | DING Wei-jun | Department of Radiation Oncology, Taizhou Hospital, Taizhou 317000, China | | YANG Hai-hua | Department of Radiation Oncology, Taizhou Hospital, Taizhou 317000, China | | WANG Xu-feng | Department of Radiation Oncology, Taizhou Hospital, Taizhou 317000, China | | 胡炜 | Department of Radiation Oncology, Taizhou Hospital, Taizhou 317000, China | | 雷皓 | 中国科学院波谱与原子分子物理国家重点实验室 | | 李春霞 | 中国科学院波谱与原子分子物理国家重点实验室 | | 方芳 | 中国科学院波谱与原子分子物理国家重点实验室 | | 方周溪 | 温州医学院电镜室 |
|
| Hits: 2939 |
| Download times: 1872 |
| Abstract:: |
| Objective To study the relationships between dynamic changes of the hippocampus metabolites, cognitive impairment and ultrastructural changes of hippocampus in rats during the initial 4 weeks after 6 MV X-ray whole-brain irradiation.Methods 65 rats were randomly divided into four groups as sham control (n=5), 10 Gy, 20 Gy and 30 Gy groups (n=20). The learning and memory ability was measured with the Y maze test 4,8 weeks,2,6 months after irradiation. 1H-MRS was performed after 2 or 4 weeks'brain irradiation. The ultrastructural changes of the hippocampus were observed by electronic microscope.Results The learning and memorizing ability of irradiation groups was significantly different from that of control group. Compared with control group, the NAA/Cr and Cho/Cr ratio in the left hippocampus in 10 Gy, 20 Gy and 30 Gy groups at 2 weeks and 4 weeks decreased significantly. Neuronal mitochondria edema, endothelial cells swelling and lamina dissociation in myelin sheath were demonstrated in various degrees by electromicroscope at 4 weeks following whole brain irradiation.Conclusions 1H-MRS can be used to non-invasively monitor the metabolic changes, both quantitatively and dynamically, of the irradiated rat brain. 1H-MRS is superior to MRI in detecting early abnormality of the brain. The NAA/Cr and Cho/Cr ratio in irradiated hippocampus could reflect the severity of the brain injury to some extent. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
