| GUO Zhi-ying,TIAN Mei,LIU Jian-xiang.Experimental study of gene expression in lung and bronchus of radon-exposed mice[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):335-338 |
| Experimental study of gene expression in lung and bronchus of radon-exposed mice |
| Received:March 27, 2008 |
| DOI： |
| KeyWords:Mouse Radon and its progeny Exposure Suppression subtractive hybridization Subtracted cDNA library |
| FundProject:科技部科研院所社会公益研究专项(2005DIB1J087) |
| Author Name | Affiliation | E-mail | | GUO Zhi-ying | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | TIAN Mei | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | LIU Jian-xiang | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 阮健磊 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 朴春南 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 苏旭 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To construct and identify differentially expressed cDNA library in lung and bronchus of mice exposed to radon.Methods 2 week old, weighing (18-22)g, male BALB/c mice were placed in a SR-NIM02 radon chamber. One group of mice was exposed to radon, which was equivalent to the accumulative dose of 30 WLM. The control group was about 0.02 WLM. To construct a subtracted cDNA library enriched with differentially expressed genes, the Super SMART technique and the suppression subtractive hybridization (SSH) were performed. The obtained forward and reverse cDNA fragments were directly inserted into pGEM-T-easy vector and transformed into E. coli DH5α. The inserts in plasmid were amplified by nested polymerase chain reaction (PCR), and some of which were sequenced. In the end these sequences were BLASTed with GeneBank.Results 146 of 460 clones obtained randomly were positive clones contained (1000-1500)bp inserted cDNA fragments. The forward and reverse subtracted cDNA library in lung and bronchus of mice exposed to radon was constructed, and 48 up-regulation and 61 down-regulation cDNA sequences selected were homologous with GeneBank in different extent.Conclusions The subtracted cDNA library in lung and bronchus of mice exposed to radon is successfully constructed, and genes that differentially expressed are identified. Some genes might have relation with the immunity, cell cycle and apoptosis. |
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