| SHENG Li-ming,SHUI Yong-jie,ZHONG Xian.Radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 human lung adenocarcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):323-326 |
| Radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 human lung adenocarcinoma cells |
| Received:December 29, 2007 |
| DOI： |
| KeyWords:Lung cancer Radiotherapy COX-2 inhibitor Radiosensitization |
| FundProject:国家自然科学基金资助项目(30470501) |
| Author Name | Affiliation | E-mail | | SHENG Li-ming | Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang University School of Medicine, Hangzhou 310009, China | | | SHUI Yong-jie | Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang University School of Medicine, Hangzhou 310009, China | | | ZHONG Xian | Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang University School of Medicine, Hangzhou 310009, China | | | 沈俐 | Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang University School of Medicine, Hangzhou 310009, China | | | 魏启春 | Department of Radiation Oncology, The Second Affiliated Hospital, Cancer Institute, Zhejiang University School of Medicine, Hangzhou 310009, China | Qichun-Wei@zju.edu.cn |
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| Abstract:: |
| Objective To investigate the radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM_1685 on A549 cells in vitro.Methods A549 human lung adenocarcinoma cell line was used in this study. Cell growth kinetics was determined using MTT assay. Cell survival was analyzed by clonogenic assay. The change of cell cycle was measured by flow cytometry.Results LM-1685 inhibited the growth of A549 cells, showing a dose-dependent and time-dependent manner. LM-1685 (50 μmol/L), either with or without IL-1β, showed the radiosensitizing effects on A549 cells, and the sensitizing enhancement ratio (SER) was 1.12 and 1.06, respectively. LM-1685 (50 μmol/L) abrogated radiation-induced G2/M arrest of the tested A549 cells.Conclusions Cyclooxygenase-2 selective inhibitor can enhance the radiosensitivity of A549 cell line. Abrogation of radiation-induced G2/M arrest could be part of the mechanism. |
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