| QIN Song-bing,WANG Li-li,TU Yu.Experimental early stage lesions after whole brain irradiation in rats: 1HMRS in comparison with NSE and S-100 protein[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):319-322 |
| Experimental early stage lesions after whole brain irradiation in rats: 1HMRS in comparison with NSE and S-100 protein |
| Received:December 20, 2007 |
| DOI： |
| KeyWords:Radiation effects Brain injuries 1HMRS NSE S-100 protein |
| FundProject:国家自然科学基金资助项目(30670638) |
| Author Name | Affiliation | E-mail | | QIN Song-bing | Department of Radiation Oncology, The First Affiliated Hospital to Soochow University, Suzhou 215006, China | | | WANG Li-li | Department of Radiation Oncology, The First Affiliated Hospital to Soochow University, Suzhou 215006, China | | | TU Yu | 放射医学与公共卫生学院 | tuyu163@163.com | | 周菊英 | Department of Radiation Oncology, The First Affiliated Hospital to Soochow University, Suzhou 215006, China | | | 徐晓婷 | Department of Radiation Oncology, The First Affiliated Hospital to Soochow University, Suzhou 215006, China | | | 沈海林 | 放射医学与公共卫生学院放射科 | |
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| Abstract:: |
| Objective To study the value of 1H magnetic resonance spectroscopy (1HMRS) changes and the expression of NSE and S-100 protein in Sprague-Dawley (SD) rat brain tissue irradiated by 6 MeV electron beam.Methods Forty adult SD rats were randomly divided into 2 groups: blank control and irradiation groups. The irradiation group was irradiated the whole brain of wat with a dose of 20 Gy using 6 MeV electron beam. Then the rats were performed with 1HMRS on days 1,7,14 and 30 after radiation to analyze concentration changes of NAA, Cho and Cr, as compared with integra-area to calculate NAA/Cr and Cho/Cr. Immunohistochemical method was used to measure the contents of S-100 and NSE in brain tissue. Results The expression of S-100 and NSE and the ratio of NAA/Cr and Cho/Cr after whole brain irradiation were time-dependent. Compared with those of control group, the indexes above were changed with the different dose levels at different times after radiation [F=19.6(NAA/Cr), 15.32(Cho/Cr),35.15(NSE) and 17.45 (S-100),P<0.05].Conclusions The changes of NAA/Cr,Cho/Cr,NSE and S-100 on the brain can act as the indexes of acute brain injury induced by radiation. 1HMRS can be used to diagnose the injury of the brain in early stage after whole brain irradiation. |
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