| JU Gui-zhi,YAN Feng-qin,FU Shi-bo,et al.Effect of RNAi p21 gene on uncoupling of EL-4 cells induced by X-irradiation[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):315-318 |
| Effect of RNAi p21 gene on uncoupling of EL-4 cells induced by X-irradiation |
| Received:October 28, 2007 |
| DOI： |
| KeyWords:X-irradiation RNAi p21 Polyploid Uncoupling |
| FundProject:国家自然科学基金资助项目(30670630) |
| Author Name | Affiliation | E-mail | | JU Gui-zhi | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | jugz@mail.jlu.edu.cn | | YAN Feng-qin | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | FU Shi-bo | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 沈波 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 孙世龙 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 杨英 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | 李鹏武 | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To investigate the effect of RNAi p21 gene on uncoupling of EL-4 cells induced by X-irradiation.Methods Construction of RNAi p21 plasmid of pSilencer3.1-H1 neo-p21 was performed. Lipofectamine transfection assay was used to transfer the p21siRNA into EL-4 cells. Fluorescent staining and flow cytometry (FCM) analysis were employed for measurement of protein expression. Fluorescent staining of propidium iodide (PI) and FCM were used for measurement of polyploid cells.Results In dose-effect experiment it was found that the expression of P21 protein of EL-4 cells increased significantly 24 h after X-irradiation with different doses compared with sham-irradiated control. In time course experiment it was found that the expression of P21 protein of EL-4 cells increased significantly at 8 h to 72 h after 4.0 Gy X-irradiation compared with sham-irradiated control. The results showed that the number of polyploid cells in EL-4 cells was not changed markedly after X-irradiation with doses of 0.5-6.0 Gy. After RNA interference with p21 gene, the expression of P21 protein of EL-4 cells decreased significantly 24 h and 48 h after 4.0 Gy X-irradiation in transfection of plasmid of pSilencer3.1-H1 neo-p21 compared with transfection of plasmid of pSilencer3.1-H1 neo control. And at the same time, the number of polyploid cells in EL-4 cells was increased significantly in transfection of plasmid of pSilencer3.1-H1 neo-p21 compared with transfection of plasmid of pSilencer3.1-H1 neo control.Conclusions Uncoupling could be induced by X-irradiation in EL-4 cells following RNAi p21 gene, suggesting that P21 protein may play an important role in uncoupling induced by X-rays. |
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