| WANG Ji-dong,WANG Jun-jie,ZHAO Yong.Relative biological effectiveness of 125I seeds for low-dose-rate irradiation of PANC-1[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):252-255 |
| Relative biological effectiveness of 125I seeds for low-dose-rate irradiation of PANC-1 |
| Received:June 15, 2007 |
| DOI： |
| KeyWords:Low-dose-rate 125I seeds Relative biological effectiveness Pancreatic cancer Cell stain |
| FundProject: |
| Author Name | Affiliation | | WANG Ji-dong | Cancer Center, Third Hospital of Peking University, Beijing 100083, China | | WANG Jun-jie | Cancer Center, Third Hospital of Peking University, Beijing 100083, China | | ZHAO Yong | 中国科学院动物研究所生物膜与膜生物国家重点实验室 | | 庄洪卿 | Cancer Center, Third Hospital of Peking University, Beijing 100083, China | | 廖安燕 | Cancer Center, Third Hospital of Peking University, Beijing 100083, China |
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| Abstract:: |
| Objective To investigate the relative biological effectiveness(RBE) of National Model 6711 125I seeds and the response patterns of PANC-1 exposed to 125I seeds irradiation. Methods PANC-1 cells in exponential growth were irradiated at initial dose rate of 2.59 cGy/h in vitro and exposed to 1, 2, 4, 6, 8 and 10 Gy. Meanwhile, the other part of cells were exposed to the same doses by 60Co at dose rate of 2.21 Gy/min. After irradiation, the cells were stained by trypan blue to measure the cellular mortality rate and to compare the changes along with plating times of 12, 24, 48 and 72 h after 4 Gy.The colonies were counted to obtain the plating efficiencies by colony-forming assay and the cell surviving fraction was calculated to plot cell survival curves, and RBE of 125I seeds relative to 60Co was determined. Results The cell death rate for continuous low-dose-rate (LDR) irradiation by 125I seeds was greater than 60Co at the same doses above or equal to 4 Gy. After 4 Gy irradiation, the cellular mortality rates were increased with times. The difference was significant between 125I seeds and 60Co. The survival fractions of 125I were lower than those of 60Co, and the RBE of 125I relative to 60Co was determined to be 1.45. Conclusion The cell-killing effects for continuous low-dose-rate (LDR) irradiation by 125I seeds are greater than acute high-dose-rate of 60Co. |
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