| ZOU Hua-wei,TAN Yong-gang,ZHANG He-ying.Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549[J].Chinese Journal of Radiological Medicine and Protection,2008,28(3):249-251 |
| Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549 |
| Received:September 03, 2007 |
| DOI： |
| KeyWords:X-rays Lung cancer cell stain p57kip2 TGF-β1 |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZOU Hua-wei | Department of Oncology, Shengjing Hospital, China Medical University, Shenyang, 110004, China. | zouhuawei@medmail.com.cn | | TAN Yong-gang | Department of Oncology, Shengjing Hospital, China Medical University, Shenyang, 110004, China. | | | ZHANG He-ying | Department of Oncology, Shengjing Hospital, China Medical University, Shenyang, 110004, China. | |
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| Abstract:: |
| Objective To analyze the effect of X-irradiation on the proteins expression of p57kip2 and TGF-β1 in lung cancer cell stain A549 and its clinical significance. Methods Lung cancer cell stain A549 was cultivated and cell, protein was extracted at 6,12,24,36 and 48 hours after X-irradiation by different doses(2,4,8 and 12 Gy). The expression of p57kip2 and TGF-β1 proteins were examined by Western blot. Results The expression of p57kip2 in lung cancer cell stain A549 was very low before X-irradiation, and increased significantly after irradiation with different doses and reached the peak level at 12 hours after irradiation(P<0.05). TGF-β1 reached its peak level at 6 hours after irradiation(P＜0.05). Conclusions X-irradiation can up-regulate the expression of p57kip2 and TGF-β1 proteins which increased with certain doses. p57kip2 and TGF-β1 could be used to predict the damage degree of cancer cells by X-ray. |
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